中文摘要：

目的：探查伤寒沙门菌hfq基因在高渗应激早期对其他基因表达的影响。方法：用自杀质粒介导的同源重组方法制备伤寒沙门菌hfq基因缺陷变异株；利用伤寒沙门菌全基因组芯片技术，比较伤寒沙门菌野生株和咆向基因缺陷变异株在高渗应激早期的基因表达谱差异，并选择部分表达差异基因进行实时定量PCR验证。结果：伤寒沙门菌hfq基因缺陷变异株制备成功；基因表达谱比较分析结果表明伤寒沙门菌hfq基因缺陷变异株在高渗应激早期有62个基因表达上调，有32个基因表达下调。实时定量PCR结果与芯片分析一致。结论：Hfq作为一个调节因子在伤寒沙门菌高渗应激早期对基因表达起重要调节作用。

英文摘要：

Objective： To explore the influence of Hfq on gene expression regulation of S. enterica serovar Typhi at early-stage of hyperosmotic stress. Methods： The hfq deleted mutant of S. enterica serovar Typhi was prepared by homologous recombination mediated by suicide plasmid; the difference of gene expression profiles between the wild strain and the hfq mutant at early-stage of hyperosmotic stress was investigated by genomic assay; qRT-PCR was performed to validate the results of microarray assay. Results： The hfq deleted mutant of S. enterica serovar Typhi was constructed successfully ; gene expression profiles analysis revealed that expression of 62 genes and 32 genes were induced and decreased respectively in the hfq mutant at earlier stage of hyperosmotic stress. The results of qRT-PCR were consistent with the results of genomic assay. Conclusion： Hfq of S. enterica serovar Typhi can serve as an important factor to regulate gene expression in response to hyperosmotic stress.