中文摘要：

为了研究大豆生殖器官发育的分子机制,前期工作中我们利用基因芯片技术进行了大豆花发育相关基因的鉴定,其中一个花优势表达基因在大豆花中的表达量为叶片中的85倍。通过生物信息学方法,拼接了该基因的全长序列,并通过RT-PCR克隆了该基因。BLAST检索分析表明该基因编码醛脱氢酶,命名为GmALDH3-1。GmALDH3-1包含一个1485bp的开放阅读框,编码494个氨基酸残基。GmADLH3-1与白杨醛脱氢酶PtALDH3相似性最高（氨基酸相似率83%,一致率为68%）,而与来自于人的HsALDH3B的氨基酸一致率和相似率分别为39%和59%。系统发生分析表明,GmALDH3-1与其他植物ALDH3亚家族成员位于一个分支,且与白杨PtALDH3和拟南芥AtALDH3F1亲缘关系最近。采用实时定量RT-PCR检测了GmALDH3-1基因在大豆叶、根和花中的表达,表明GmALDH3-1基因在花中高丰度表达,在根和叶中未检测到表达。运用基因芯片信息分析了GmALDH3-1在种子发育过程中的表达情况,表明GmALDH3-1在种子发育过程中的外表皮、内表皮、外胚珠和种脐中表达量较高。

英文摘要：

To research the mechanism of soybean reproductive development,we identified a number of flower development related genes in soybean by microarray hybridization.A gene predominately expressed in soybean flowers was chosen for further analysis.Through bioinformatic and RT-PCR approaches,the full-length gene was cloned from soybean flowers.The results of BLAST searching indicated that this gene encoded for an aldehyde dehydrogenase and was named as GmALDH3-1.GmALDH3-1 contains a complete open reading frame of 1485 bp in length,which encodes for a peptide of 494 amino acids.The product encoded by GmALDH3-1 shows 83% similarity and 68% identity with Populus tomentosa PtALDH3,respectively,and 39% and 59% with human ALDH3B.Phylogenetic analysis shows that GmALDH3-1 and other ALDH3 subfamily members are grouped into the same branch and GmALDH3-1 is close to PtALDH3 and Arabidopsis AtALDH3F1.Real-time RT-PCR analysis demonstrated that the highest expression level of GmALDH3-1 occurred in flowers,but the expression of this gene was almost undetectable in leaves and roots.We further analyzed GmALDH3-1 expression during the course of seed development based on publicly available microarray data and found that GmALDH3-1 was highly expressed in the seed endothelium,epidermis,outer integument,and hilum.