中文摘要：

采用基因芯片技术,从大豆中鉴定了一个荚优势表达基因。利用生物信息学的方法,拼接了该基因的全长序列,并通过RT-PCR克隆了该基因。Blast检索分析表明,该基因编码一个具有AP2结构域的转录因子,且与拟南芥DREB类蛋白TINY的氨基酸相似度最高,将该基因命名为GmTINY1。GmTINY1包含一个735bp的开放阅读框,编码244个氨基酸残基。GmTINY1与拟南芥TINY和TINY2蛋白的相似度分别为59%与62%。系统发生分析表明,GmTINY1、TINY和TINY2位于一个分支,且同属于DREB亚家族。实时定量RT-PCR检测表明,GmTINY1基因在荚中高丰度表达,在花中的表达量也较高,在根中的表达量较低,而在叶片中未检测到表达。基因芯片信息分析结果表明,GmTINY1在种子发育的子叶期的种脐部分高丰度表达。由此推论,GmTINY1基因在大豆生殖器官发育中可能发挥调控作用,可能与种子发育过程中种脐的形成有关。

英文摘要：

Plant reproductive development involves the coordination of a lot of genes encoding transcription factors. The AP2 domain transcription factors have been proved with critical roles in plant reproductive development. By microarray analysis, we identified a gene which showed higher expression in pod as 500 folds as that in leaf of soybean. The Blast searches indicated this gene encodes a dehydration responsive element binding protein （DREB）-like protein showing highest similarity to Arabidopsis TINY, therefore named as GmTINY1. By searching soybean genome and EST databases, the putative full-length cDNA sequence for GmTINY1 was in silico assembled. The GmTINY1 gene was cloned from soybean seeds at 15 DAF （days after flowering） by RT-PCR. GmTINY1 contained a complete open reading frame （ORF） of 755 bp which encoded a peptide of 244 amino acids. The predicted molecular mass and isoelctric point of GmTINY1 are 26.76 kD and 5.12, respectively. An AP2 domain and a Ser-rich domain were identified in GmTINY1 amino acid sequence by Motif Scan server. The GmTINY1 encoding product showed 59% and 62% sequence similarities with Arabidopsis TINY and TINY2, respectively. Multiple sequences alignment revealed that a highly conserved AP2 domain was present in each AP2 domain transcription factor. In this AP2 domain, it was found that a Ser66 was specifically present in GmTINY1, DREB1B, TINY and TINY2 proteins but a Cys66 in DREB1A and DREB1C proteins, indicating, like Arabidopsis TINY, TINY2 and DREB1B, GmTINY1 might be able to bind both the dehydration responsive element（DRE） and ethylene responsive element （ERE） motifs while DREB1A and DREB1C only bind DRE motif. Besides, it was found that a Ser-rich domain probably involving translational modification on GmTINY1 protein was located close to AP2 domain. The neighbor-joining phylogenetic tree showed that the AP2 domain transcription factors were grouped into four subfamilies： DREB, ERF, AP2, and RAV; and GmTINY1, TINY, and TINY2 were grouped into a branch wh