中文摘要：

目的：研究重组人粒细胞集落刺激因子（rhG-CSF）动员对供者CD4＋T细胞表面分子淋巴细胞功能相关抗原-1（LFA-1）、细胞间黏附分子-1（ICAM-1）、L-选择素（LAM-1）和人整合素-4（VLA-4）的表达及其介导的CD4＋T细胞功能的影响,探讨外周血干细胞移植过程中CD4＋T细胞免疫耐受机制。方法：使用三色荧光标记检测动员前及动员后第5天供者外周血LFA-1、ICAM-1、LAM-1和VLA-4的表达率,ELISA方法检测动员前后CD4＋T细胞分泌IFN-γ和IL-4能力,免疫磁性分选法分离纯化CD4＋T细胞,检测动员前后CD4＋T细胞对基质细胞衍生因子-1α（SDF-1α）的迁移能力和对ICAM-1的黏附能力。结果：动员前后CD4＋T细胞LFA-1（CD11a）和VLA-4（CD49d）表达率差异无统计学意义（P〉0.01）,动员前后CD4＋T细胞LAM-1（CD62L）和ICAM-1（CD54）的表达率差异均有统计学意义,动员前显著高于动员后（P〈0.01）;动员前后CD4＋T淋巴细胞向SDF-1α的迁移率差异无统计学意义（P〉0.01）;动员后CD4＋T细胞对ICAM-1的黏附率降低（P〈0.01）;动员后IL-4和IFN-γ两个细胞因子在外周血血清的浓度均降低（P〈0.01）。结论：rhG-CSF动员不影响CD4＋T细胞LFA-1和VLA-4表达及CD4＋T细胞迁移,但影响CD4＋T细胞ICAM-1和LAM-1表达以及CD4＋T细胞通过LFA-1对ICAM-1的黏附能力影响,并可能影响CD4＋T细胞分泌细胞因子IL-4及IFN-γ的功能。

英文摘要：

Objective： To investigate the mechanism of CD4＋ T cells immunotolerance during allogeneic peripheral blood stem cell transplantation by studying the impact of recombinant human granulocyte colony stimulating factor（rhG-CSF） on expression of lymphocyte function antigen-1（LFA-1）,intercellular adhesion molecule（ICAM-1）,L-Selectin（LAM-1） and very late antigen 4α（VLA-4） on CD4＋ T cells,and functions of CD4＋ T cells through these molecules during mobilization.Methods： The expression of LFA-1,ICAM-1,LAM-1 and VLA-4 of CD4＋ T cells were determined by FACS before rhG-CSF mobilization and the fifth day of mobilization.The expression of IFN-γ and IL-4 by CD4＋ T cells were examined with ELISA.At the same time,the migration and adhesion activity of CD4＋ T cells to SDF-1αand ICAM-1 were detected.Results： The expression of LFA-1 on CD4＋ T lymphocytes were both 100%.The expression of VLA-4 didn’t change markedly before and the fifth day of mobilization（P〉0.01）.The expression of LAM-1（CD62L） and ICAM-1（CD54） on CD4＋ T lymphocytes decreased during mobilization（P〈0.01）.SDF-1α induced CD4＋ T lymphocytes migration for 4 hours didn’t change markedly during mobilization（P〉0.10）.The adhesion activity of CD4＋T lymphocytes to ICAM-1 decreased markedly during mobilization（P〉0.10）.Conclusion： The concentration of IL-4 and IFN-γ in peripheral blood serum decreased markedly during mobilization（P〈0.01）.In conclusions,rhG-CSF does not have effect on the expression of LFA-1 and VLA-4 on CD4＋ T cell,and the migration activity of CD4＋ T cell during mobilization,but can affect the expression of ICAM-1and LAM-1 on CD4＋ T cell,and the adhesion activity of CD4＋ T cell to ICAM-1 through LFA-1.