中文摘要：

目的：分离纯化苦参多糖,并对其进行结构、单糖组成及抗氧化性能分析。方法：采用水提醇沉法获得苦参多糖,Sevag法脱蛋白、DEAE-52纤维素柱色谱法分离纯化。采用紫外和红外光谱法对苦参多糖进行纯度及结构分析,采用1-苯基-3-甲基-5-吡唑啉酮柱前衍生HPLC法测定苦参多糖的单糖组成及其含量,并考察了SFP-1的体外抗氧化性。结果：苦参多糖经DEAE-52纤维素柱色谱分离可获得4个苦参多糖组分SFP-1、SFP-2、SFP-3及SFP-4。SFP-1组分中不含蛋白质及核酸,并具有典型的多糖吸收峰;多糖组分SFP-1主要由甘露糖、鼠李糖、半乳糖醛酸、葡萄糖,半乳糖、阿拉伯糖组成,其摩尔比为0.34∶1.00∶0.12∶1.19∶0.62∶0.19。体外抗氧化性研究表明当浓度为0.7 mg·mL^-1时,SFP-1对1,1-二苯基-2-苦基苯肼自由基清除能力达56.40%,金属螯合率达18.31%。结论：SFP-1组分是由多种单糖组成,并具有较强的抗氧化性能。

英文摘要：

Objective： To extract and purify Sophora flavescens polysaccharides（ SFP）,and investigate the structure,monosaccharide compositions and antioxidant activity. Methods： The polysaccharides were extracted from Sophora flavescens with boiling water,followed by precipitation with ethanol,removal of protein by Sevag method,and purification with ion exchange chromatography on a DEAE- 52 cellulose column. The purity and their structure were analyzed by UV and FTIR spectrometer,and the monosaccharide compositions were investigated by PMP pre-column derivatization HPLC. Furthermore,the in vitro antioxidant activity was also studied. Results： Four refined polysaccharides fractions,named as SFP- 1,SFP- 2,SFP- 3 and SFP- 4,were obtained by ion exchange chromatography on DEAE- 52 cellulose column. The major component SFP- 1 showed no signals of protein and nucleic acid,and exhibited typical signals of polysaccharides. The SFP- 1 was composed of mannose,rhamnose,galacturonic acid,glucose,galactose and arabinose in a molar ratio of 0. 34∶ 1. 00∶ 0. 12∶ 1. 19∶ 0. 62∶ 0. 19. The result of in vitro antioxidant activity showed that the scavenging rate against DPPH free radical and metal chelating rate of SFP- 1 reached 56. 40% and 18. 31%,respectively. Conclusion： The SFP- 1 was composed of a variety of monosaccharides and had a strong antioxidant activity.