采用Tenax-TA吸附剂吸附法结合气相色谱和气质联用仪,在不同处理的番茄中共分离鉴定了15种挥发性化合物.这些挥发物主要由绿叶性气味物质、萜烯类物质(包括单萜、倍半萜及其衍生物)和水杨酸甲酯等成分组成.并发现斜纹夜蛾危害与机械损伤对绿叶性气味物质具有相似的诱导作用,而萜烯类物质和水杨酸甲酯等物质在斜纹夜蛾危害植株中的含量显著高于其在健康植株与机械损伤植株中的含量.斜纹夜蛾诱导植物释放的几种挥发物,以及(E)-2-己烯-1-醇和茉莉酸甲酯对萝卜幼苗具有化感作用,表现为对萝卜幼苗生长的抑制作用.其中绿叶性气味物质((E)-2-己烯醛,(Z)-3-己烯-1-醇,(E)-2-己烯-1-醇)和茉莉酸甲酯对萝卜幼苗生长的抑制作用较强,其次为水杨酸甲酯,而芳樟醇和子丁香烯两种萜类物质的抑制作用相对较小.同时证实这些挥发物的化感活性有明显的浓度效应.
Fifteen components of the headspace volatiles from tomato plants infested by herbivore S. litura were collected with Tenax-TA trap and identified with GC-MS. These volatiles are composed of green leaf volatiles, terpenoids (including monoterpenes, sesquiterpenes and their derivatives) and methyl salicylate (MESA). Significant differences in volatile emissions were observed for plants with different treatduced plant volatiles, (E)-2-hexen-l-ol and methyl jasmonate on the seedling growth of radish were further investigated. The results showed that green leaf volatiles, (E)-2-hexenal, (Z)-3-hexen-l-ol, (E)- 2-hexen-l-ol, MeSA and methyl jasmonic acid (MeJA) significantly inhibited the seedling growth of tested plants with a dose-dependent relationship. Linalool and β-caryophyllene had a slightly inhibitory effect on the seedling growth of radish in tested concentrations.