中文摘要：

Bt 玉米是顶之一三大规模商品化的转基因的庄稼在全世界。为害虫控制的互补持久的途径，把庄稼的内长的防卫与介绍外国基因相结合，为害虫抵抗管理和昆虫抵抗的转基因的庄稼的下一代正在答应其他的策略，是逐渐地清楚的。在现在的学习，我们由化学分析， ELISA，和 RT-PCR 在 Bt 玉米栽培变种 34B24 和 non-Bt 栽培变种 34B23 的叶子在直接防卫的化学内容， Bt 蛋白质集中，和相关基因表示上测试了外长的 jasmonic 酸(JA ) 的可诱导的效果。结果证明在对待的叶(第一片叶) 的哈鱼， PR-2， MPI，和 PR-1 基因的表示被外长的 JA 在 34B24 和 34B23 两个都支持。作为与控制相比，在对待的叶的 DIMBOA 的集中被 63 和 18% 显著地分别地为 34B24 和 34B23 增加。全部的酚酸被 24 和 12% 也为 34B24 和 34B23 增加。在对待的叶的 34B24 的 Bt 蛋白质内容被 13% 增加，但是在第二片叶在 27% 显著地减少了。34B24 的导致的反应以一个全身的方法并且比 34B23 的强壮得多。那些调查结果显示在 Bt 之间有基因和内部导致的化学防卫系统在 Bt 玉米由外部地应用的 JA 触发了的一个 synergistic 相互作用。

英文摘要：

Bt corn is one of the top three large-scale commercialized transgenic crops around the world. It is increasingly clear that the complementary durable approaches for pest control, which combine the endogenous defense of the crop with the introduced foreign genes, are promising alternative strategies for pest resistance management and the next generation of insect-resistant transgenic crops. In the present study, we tested the inducible effects of exogenous jasmonic acid （JA） on direct-defense chemical content, Bt protein concentration, and related gene expression in the leaves of Bt corn cultivar 34B24 and non-Bt cultivar 34B23 by chemical analysis, ELISA, and RT-PCR. The results show that the expression of LOX, PR-2a, MPI, and PR-I genes in the treated leaf （the first leaf） was promoted by exogenous JA both in 34B24 and 34B23. As compared with the control, the concentration of DIMBOA in the treated leaf was significantly increased by 63 and 18% for 34B24 and 34B23, respectively. The total phenolic acid was also increased by 24 and 12% for both 34B24 and 34B23. The Bt protein content of 34B24 in the treated leaf was increased by 13% but decreased significantly by 27% in the second leaf. The induced response of 34B24 was in a systemic way and was much stronger than that of 34B23. Those findings indicated that there is a synergistic interaction between Bt gene and internally induced chemical defense system triggered by externally applied JA in Bt corn.