中文摘要：

【目的】Bt（Bacillus thuringiensis）玉米（Zea may sL.）是全球商品化程度最快的抗虫转基因作物之一,协调利用其内在防御机制和人工导入抗性是目标害虫抗性风险综合管理和第2代抗虫转基因作物培育的主要策略之一。【方法】采用基因表达分析、化学物质分析和ELISA蛋白定量方法,研究外施信号物质茉莉酸对Bt玉米34B24（Mon810）及其同源常规玉米34B23幼苗叶片直接防御物质含量及其相关基因表达的影响。【结果】外源茉莉酸处理能诱导34B24和34B23处理叶（第1叶）中LOX、PR-2a、MPI和PR-1基因的表达,第1叶丁布含量分别比对照增加63%和18%、总酚含量分别增加24%和12%。茉莉酸处理使34B24第1叶中的Bt蛋白含量增加了13%,但第2叶中的显著降低了27%。与34B23相比,茉莉酸对34B24的诱导作用更强,并具有系统性。【结论】人工外施茉莉酸的条件下,Bt玉米人工导入抗性（Bt基因）和自身化学防御过程之间的互作关系是协同的。

英文摘要：

[Objective] Bt corn is one of the top three large-scale commercialized transgenic crops around the world. It is increasingly clear that the complementary durable approaches for pest control, which combine the endogenous defense of the crop with the introduction of foreign genes, are promising alternative strategies for pest resistance management and the next generation of insect-resistant transgenic crops. [Method] In the present study, we tested the inducible effects of exogenous jasmonic acid （JA） on direct defense chemicals content, Bt protein concentration and related genes expression in the leaves of Bt corn cultivar 34B24 and Non-Bt cultivar 34B23 by chemical analysis, ELISA and RT-PCR. [ Result] The expression of LOX, PR-2a, MPI and PR-1 genes in the treated leaf （first leaf ） was promoted by exogenous JA both in 34B24 and 34B23. As compared with the control, the concentration of DIMBOA in the treated leaf significantly increased by 63% and 18% in 34B24 and 34B23, respectively. The total phenolic acid also increased by 24% and 12% in 34B24 and 34B23 either. The Bt protein content of 34B24 in the treated leaf increased by 13%, but decreased significantly by 27% in the second leaf. The induced response of 34B24 is in a systemic way and much stronger than that of 34B23.[Conclusion]These findings indicated that there was a synergistic interaction between Bt gene and jasmonic-acid-mediated induced defense in Bt corn.