中文摘要：

随着纳米科技的发展和纳米材料的广泛应用，进入环境中的纳米材料必将随之增加。由于纳米材料可能对生态环境和人体健康带来潜在的危害，了解环境中纳米材料的浓度水平和行为有助于正确认识和评价纳米材料的环境风险，并做出相应的防治对策。然而，纳米材料所具有的诸多特殊性质直接影响它们的环境行为和生态毒性，因此，环境中纳米材料的分析不仅包括浓度的定量测定，还包括对其组成、粒径和表面电荷等性质的表征，是一项具有挑战性的工作。本文综述了近年来关于环境纳米材料分离、表征及检测的相关手段和方法，重点包括场流分离、色谱分离和电泳分离在纳米材料分离以及显微和谱学技术在纳米材料分析中的应用；提出了一些思考和展望，希望能够增进对环境中纳米材料分离和分析方法的了解，有助于纳米材料环境分析方法的建立和推动纳米材料环境行为和生态毒理研究的进一步规范。

英文摘要：

With the rapid development of nanotechnology and the wide application of manufactured nanomaterials, the potential for their release into the environment will increase drastically in the near future. Studies have suggested that the released nanomaterials can cause some potential adverse effects on both ecosystem and human health. Therefore, knowledges of concentration, behavior, and fate of nanomaterials in the environment will help us to accurately evaluate their environmental risks in order to make proper prevention and control countermeasures. However, due to the fact that many properties will influence the behavior and toxicity of nanomaterials in the environment, analysis of nanomaterials includes not only the determination of their concentration but also the identification of their characteristics such as composition, size, surface charge etc. , therefore posing a great challenge to accurate analysis of nanomaterials in the environment. This review presents the update methods used for extraction, separation, fractionation and analysis of nanomaterials in the environment, mainly including extraction and preseparation procedures, field flow, chromatographic and electrophoretic methods used for fractionation, and microscopic and spectroscopic techniques applied in characterization and quantification of nanomaterials. It concludes with future research perspectives of these methods, which will hopefully help to establish normative methods and strategies for environmental analysis and improve the ecological risk assessment of nanomaterials in the environment.