中文摘要：

目的：探讨蝶啶荧光分析法用于绯颜裸金蝇Achoetandrus rufifacies成虫日龄及进一步用于死亡时间推断的潜力。方法：将A.rufifacies成虫于16、20、24、28和32℃5种恒温下饲养,此后取样1次/2~3 d。取各日龄雌、雄成虫头壳于Tris-HCl（pH8.0）缓冲液中匀浆,离心,用荧光分光光度计测定上清液的荧光强度。结果：在5种恒温下,雌、雄成虫头壳蝶啶含量与日龄均呈显著的线性关系（r2〉0.87,P〈0.01）;蝶啶积累速率与温度也呈显著的线性关系（r2〉0.87,P〈0.01）。应用蝶啶荧光分析法对自然温度下A.rufifacies雌、雄成虫日龄的估计表明,估计与实际日龄呈显著的线性关系（r2〉0.52,P〈0.01）,其中雌成虫日龄估计误差平均为±3.6 d,雄成虫±2.3 d。结论：蝶啶荧光分析法可有效用于A.rufifacies成虫日龄的推断。

英文摘要：

Objective： To explore the method for determining the age of adult Achoetandrus ruff.facies by pteridine tluo- reseenee analysis. Methods： Adult A. rufifacies were reared at 16, 20, 24, 28 and 32 ℃ and sampled every 2 or 3 days. Head capsules of the adult samples were homogenized in 3 mL Tris-HCl（pH 8.0）buffer, centrifuged and analyzed using speetrofluorometer. Results： Significant linear relationships were found between ptefidine fluoreseenee and age for the adults maintained at 5 constant temperatures（ r^2 〉 0.87, P 〈 0.01）. The relationships between the rate of pterldine accumulation and temperature were well-described by linear equations for female and male adults （ r^2 〉 0.87, P 〈 0.01）. The age of another colony of adult A. rufifacies maintained at ambient temperature was determined by pteridine fluorescence analysis. A significant linear relationship between estimated age and chronological age was found for female or male adults（r^2 〉 0.52, P 〈0.01）. The average error of the estimated age was ±3.6 d in females and ±2.3 d in males. Conclusion： Pteridine fluorescence analysis is a valuable method for determining the age of adult A. rufifacies, especially for males.