中文摘要：

用激活标签载体pSK1015和农杆菌介导的浸花转化法创建拟南芥激活标签突变体，优化了浸花法转化方案，采取原地转化的措施，减少了工作量，又保证了植株在转化期间的正常生长，转化效率高达1．434％．突变体分析表明，57．5％含单拷贝T-DNA插入，100%整合了Bar基因，87％含有CaMV35S增强子，进一步分析发现CaMV35S增强子丢失机率与农杆菌继代次数呈正相关。

英文摘要：

To increase transformation efficiency, we modified the procedure of floral dipping method, and transformed plants at the original site during the generation of acitation tagging mutants library of Arabidopsis thaliana. This modification reduced workload, guaranteed the normal growth of plants and achieved relatively high transformation efficiency up to 1.434%. The mutant library was analyzed by detecting T-DNA copy number or the enhancer genes and Bar gene. The result showed that 57.5% mutants contained single-copy T-DNA, 100% harbored Bar gene and 87% were activation-tagged. Further analysis showed that the rate of loss of CaMV 35S enhancers was positively correlated to the times of culturing Agrobacterium tumefaciens.