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Isolation and identification of Sclerotinia stem rot causal pathogen in Arabidopsis thaliana
  • ISSN号:1673-1581
  • 期刊名称:《浙江大学学报:B卷英文版》
  • 时间:0
  • 分类:Q949.32[生物学—植物学]
  • 作者机构:[1]Computer and Information College, Fujian Agriculture and Forestry University, Fuzhou 350002, China, [2]Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • 相关基金:Project supported by the National Natural Science Foundation of China (No.30471178) and the Ministry of Agriculture of China (No. nyhyzx07-054)
中文摘要:

新茎腐烂疾病被发现在 Fuzhou 的温室在 Arabidopsis 植物上自然地发生,中国。识别它的病原体的无异状,我们进行了从 sclerotia 的一系列真菌的隔离和纯化,植物接种,和子囊和 ascospore 正式就职。在接种以后引起典型 water-soaked 损害并且在 Arabidopsis 植物和文化媒介盘子上两个都生产了 sclerotia 的 isolate,和 sclerotia 能被导致生产 discal apothecia 和 8 双性人起核心作用 ascospores 每子囊。这些疾病症状和真菌的形态学数据表明真菌 Sclerotinia sclerotiorum (解放) de Bary 是为 Arabidopsis 的病原体茎腐烂。证实这,我们进一步由聚合酶链反应(PCR ) 放大了它的大子单元 ribosomal DNA (LSU rDNA ) ,并且在 GenBank 把顺序与已知的 LSU rDNA 序列作比较。结果证明顺序与不同 S 的 LSU rDNAs 分享最高的身份。sclerotiorum 种类。一起拿所有这些数据,我们断定引起了 Arabidopsis 茎的真菌腐烂是 S。sclerotiorum (解放) de Bary。这是 Arabidopsis 被 S 自然地感染的第一份报告。sclerotiorum。

英文摘要:

A new stem rot disease is found to occur naturally on Arabidopsis plants in greenhouses of Fuzhou, China. In order to identify its pathogen, we conducted a series of fungal isolation and purification, plant reinoculation, and ascus and ascospore induction from the sclerotia. The isolate caused typical water-soaked lesions after reinoculation and produced sclerotia both on Arabidopsis plants and culture medium plates, and the sclerotia could be induced to produce discal apothecia and 8 binucleate ascospores per ascus. These disease symptom and fungal morphology data revealed that the fungus Sclerotinia sclerotiorum (Lib.) de Bary was the pathogen for Arabidopsis stem rot. To confirm this, we further amplified its large subunit ribosomal DNA (LSU rDNA) by polymerase chain reaction (PCR), and compared the sequence with the known LSU rDNA sequences in GenBank. The results show that the sequence shares the highest identities with the LSU rDNAs of different S. sclerotiorum strains. Taking all these data together, we concluded that the fungus that caused the Arabidopsis stem rot is S. sclerotiorum (Lib.) de Bary. This is the first report that Arabidopsis is naturally infected by S. sclerotiorum.

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期刊信息
  • 《浙江大学学报:B卷英文版》
  • 中国科技核心期刊
  • 主管单位:
  • 主办单位:浙江大学
  • 主编:
  • 地址:杭州玉古路20号,浙江大学学报《英文版》编辑部
  • 邮编:310027
  • 邮箱:jzus@zju.edu.cn
  • 电话:0571-87952276 87952331
  • 国际标准刊号:ISSN:1673-1581
  • 国内统一刊号:ISSN:33-1356/Q
  • 邮发代号:
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  • 被引量:323