中文摘要：

为探讨稳定转化家蚕细胞表达外源基因的新方法，以家蚕核型多角体病毒（BmNPV）极早期蛋白基因（ie-1）启动子元件驱动新霉素抗性基因（neo＇），并克隆至具有绿色荧光蛋白基因（gfp）标记的昆虫转座子载体piggyBac中，构建转基因载体pigA3-IE—Neo。以该载体转染家蚕BmN细胞，用终浓度800μg／mL的遗传霉素（geneticin，G418）筛选3个月，获得了稳定转化的细胞，呈现绿色荧光的细胞数达80％以上。通过PCR鉴定证实了细胞基因组DNA中neo基因和gfp基因的存在。

英文摘要：

Neomycin resistance gene driven by ie-1 promoter element of Bombyx mori nucleopolyhedrorvirus was cloned into insect transposon vector piggyBac, which contains gfp report gene, to form transgenic vector pigA3-1E-Neo. Then it was used to transfect BmN cell. Screening under G418 at 800 μg/mL final concentration for three months a stable transformation cell strain with over 80% fluorescent cells was obtained. Identification of PCR indicated that neo＇ and gfp genes existed in the genomic DNA of the transgenic cell strain. This study brought forward a new way to express interesting gene by using stable transformation cell.