通过PCR克隆,从家蚕基因组中获得大小为975bp的DNA片段(登录号:EU352872),Blast搜寻结果显示,该片段DNA序列的52-449nt区域与野蚕mariner-like元件DNA(登录号:AB237562)的同源性达90%,推测为家蚕类mariner转座子相关序列,在乙酰胆碱酯酶基因的第5内含子、微卫星S1104-R序列、BmBRC-NZ3mRNA终止码的下游序列、ErB.1基因的内含子均检测到家蚕类mariner元件相关序列的同源区,暗示家蚕类mariner元件在家蚕基因组中发生了多次转座,并在家蚕基因组的不同区域随着进化发生了歧化。
A DNA fragment of 975bp sequence (GenBank accession No: EU352872), which may be a mariner-like elements associated sequence, was acquired form Bombyx mori genomic DNA by PCR amplification. Blast search showed that there is 90% homology between 52-449nt regions of this sequence and Bombyx mandarina mariner-like elements DNA, its homologous sequence was also found in the 5th intron of acetylcholinesterase gene, microsatellite S1104-R sequence, downstream sequence of stop codon of BmBRC-NZ3 mRNA and intron of ErB. 1 gene. It was implied that the mariner-like elements associated sequence was transposed time after time in the Bombyx mori genomic DNA, and was diverged in different regions of genomic DNA during evolutional process.