中文摘要：

目的研究大骨节病有关病因因素NIV毒素和硒对软骨细胞外基质蛋白聚糖代谢的损伤和保护作用；探索其引起软骨细胞变性坏死的机制。方法在体外单层培养的人胚软骨细胞中加入NIV毒素和硒，5d后收集软骨细胞和细胞培养液，利用半定量RT—PCR方法检测蛋白聚糖核心蛋白mRNA在软骨细胞中的转录情况；利用咔唑一硫酸法检测软骨细胞培养液中葡萄糖醛酸水平。结果NIV毒素使软骨细胞中蛋白聚糖核心蛋白mRNA水平明显下降，毒素培养液中葡萄糖醛酸的浓度明显增加。毒素加硒组与毒素组变化趋势一致，但数值略下降。组间差异有统计学意义（P〈0．05）。结论NIV毒素能从转录水平抑制软骨细胞蛋白聚糖的合成，加速蛋白聚糖降解，造成基质中蛋白聚糖代谢紊乱，关节软骨受到损伤。补硒可以在一定程度上拮抗NIV毒素对软骨细胞的毒性作用，但保护作用有限，不能从根本上防止损伤的发生。

英文摘要：

Objective To investigate the effect of nivalenol and selenium on the metabolism of aggrecan in the cultured chondrocytes, and to explore the mechanism involved in cartilage aggrecan catabolism in the process of kashin- beck disease. Method Aggrecan mRNA expression was studied by RT- PCR. The concentration of GlcUA in culture medium was determined by diphenyleni- mine- sulfuric acid method. Result NIV significantly decrease aggrecan mRNA expression. That selenium can partially antagonize the effect of NIV on aggrecan mRNA expression. The content of GlcUA in medium with NIV was higher than that in other groups. Conclusion NIV could inhibit chondrocyte synthesis aggrecan, promote the loss of aggrecan from cartilage. All the effects result in the metabolic disorder of the cartilage aggrecan, which eventually leads to irreversible mechanical destruction of the cartilage. It suggested that selenium can partially alleviate the effects of NIV on chondrocytes cultured in vitro.