中文摘要：

制备了四种由β-四（3,5-二羧基苯氧基）酞菁锌（记为1）和β-八（3,5-二羧基苯氧基）酞菁锌（记为2）与人血清白蛋白（HSA）或牛血清白蛋白（BSA）构成的共价结合物,结合物中酞菁与白蛋白的摩尔组成比为6-7∶1。在PBS溶液中的电子吸收光谱测试表明,当酞菁1共价结合到白蛋白上后,呈现出更明显的单体特征吸收（单体特征吸收峰位于677 nm）;而酞菁2在白蛋白网络中,主要以有利于光动力治疗的单体形式存在,最大吸收波长位于681 nm附近,摩尔吸光系数分别为2.01×10^5mol-1.L.cm-1（2-BSA）和2.05×10^5mol-1.L.cm-1（2-HSA）。两种酞菁被固定在白蛋白上后,在水溶液中的Q带吸收光谱和存在状态不随体系pH值的变化而改变,这与相应的游离酞菁明显不同。

英文摘要：

Four kinds of covalent serum albumin（BSA or HSA） conjugates of β-tetrakis[（3,5-dicarboxy）phenoxy]-phthalocyaninatozinc（1） and β-octakis [（3,5-dicarboxy）phenoxy]-phthalocyaninatozinc（2） were synthesized by the approach of amide bond.The molar ratio of phthalocyanine to albumin in conjugates were found to be 6~7∶1.Their absorption spectra were measured in PBS solution.When conjugated to albumin,compound 1 displays more distinct monomer absorption characteristics（with the maximum absorption at 677 nm） than its free form.Compound 2 in albumin framework exists mostly in monomer form,which is beneficial to photodynamic therapy in aqueous solution.Both 2-BSA and 2-HSA show a sharp and intense Q-band at 681 nm and 682 nm with the molar extinction coefficient of 2.01×10^5 and 2.05×10^5 mol-1·L·cm-1 respectively.The Q-band absorption spectra and existent state of phthalocyanine 1 or 2 in conjugates were not affected by the pH value of aqueous solution,which are obviously different from that of the corresponding free phthalocyanine.