中文摘要：

本文初步研究报道了红松胚性愈伤组织的超低温保存方法，结果表明，预处理、保护剂与解冻方式对胚性愈伤组织存活率均有一定影响。效果较好的方法为：利用0．4mol·L^-1 Sorh（山梨醇）预处理愈伤组织24h，5％DMSO（二甲基亚砜）＋0．4mol·L^-1 Sorb为冷冻保护剂，冷冻方式为程序降温盒在-70℃停留3h后投入液氮罐中保存，解冻方式为20℃流水冲洗。胚性愈伤组织解冻后再培养，其存活率可达71．4％。

英文摘要：

Preliminary study on cryopreservation of Korean pine somatic calli showed that the pretreatment, the cryoprotectant and thawing method had certain influences on the survival rate of calli. The better-performing method was established： calli were pretreated with 0.4 mol·L^-1 Sorb for 24 h, and then kept in programmed cooling box Nalgene Mr. Frosty at -70 ℃ for 3 h with 5 % DMSO and 0.4 mol·L^-1 Sorb as cryoprgtectant, at last put into liquid nitrogen container. While thawed in 20 ℃ flowing water, the survival rate of thawed calli was 71.4%.