中文摘要：

以甲酸为溶剂，将具有特定信号识别功能的RGD重组蛛丝蛋白（pNSR32）与聚己内酯（PCL）和壳聚糖（CS）共混，采用静电纺丝技术制备复合纳米纤维小直径血管支架，扫描电子显微镜（SEM）观察支架材料的微观结构，并通过溶血率、动态凝血时间、血小板黏附以及复钙化凝血时间实验评价支架材料的血液相容性。研究表明，（1）所制备的pNSR32／PCL／CS复合纳米纤维支架内径为4mm，长度可达8cm，并且纤维连续，孔隙相通，孔隙率达85％以上，支架具有三维多孔网状结构，有利于种子细胞在支架上的黏附、增殖和迁移；（2）复合支架材料的溶血率〈5％，黏附的血小板少且不变形，复钙化凝血时间达293s，与单独PCL相比延长94s，说明该支架材料具有良好的血液相容性，有望成为一种新型的小直径组织工程血管支架。

英文摘要：

The composite nano-fiber small diameter vascular scaffold using recombinate spide silk protein with RGD（pNSR32）,poly（ε-caprolactone） （PCL） and chitosan（CS） was prepared by elecstrospinning. The microstructures of scaffold material were observed using SEM. Additionally, hemolysis ratio, dynamic clotting time, platelet daherenee and recalcification time were carried out to evaluate the blood compatibility. These results indicate that scaffolds with 4mm inner diameter and 8cm length possess porosity and 3-diamentions stucture, which benefits for cell attachment, proliferation and migration. The hemolysis rates of the scaffold are less than 5%. The platelet adherence experiments showed there were less blood platelets sticking on the surface of the composite pNSR32/PCL/CS scaffolds,and the platelets were not deformed. The recalcification time of pNSR32/ PCL/CS is 293s,which was more 94s than PCL. The pNSR32/PCL/CS composite nano-fiber scaffolds appear to be of fine blood compatibility. The composite nano-fiber scaffolds applly to the small diameter vascular scaffold as a novel biomaterial.