中文摘要：

在 genomic DNA 的 hydroxylmethyl- 和 formylpyrimidines 的鉴定是在 epigenetics 的一个里程碑事件。相关的地里的众多的实验室正在调查这些和另外的 nucleic 酸修正的生物学。然而，处于检测并且综合的能力的限制拨出修正是妨碍。此处，我们在 5-formyluracil 的选择察觉探索了显著开发在搁浅单人赛并且在温和条件下面的双 stranded DNA。向 5-formyluracil 的打开特性在检测包含 5-formyluracil 的材料的品质上和份量上启用了高 signal-to-noise 比率，它没被 abasic 地点和 5-formylcytosine 的存在影响， 5-formyluracil 的修改 cytosine 对应物。在 5-formyluracil phosphoramidite 的摘要，无毒，便利，和费用效率，合成常规将支持这自然 thymidine 修正的 epigenetic 角色的理解。

英文摘要：

The identification of hydroxylmethyl- and formylpyrimidines in genomic DNA was a landmark event in epigenetics. Numerous laboratories in related fields are investigating the biology of these and other nucleic acid modifications. However, limitations in the ability to detect and synthesize appropriate modifications are an impediment. Herein, we explored a remarkable development in the selective detection of 5-formyluracil in both single-stranded and double-stranded DNA under mild conditions. The ＂switch-on＂ specificity towards 5-formyluracil enabled a high signal-to-noise ratio in qualitatively and quantitatively detecting materials containing 5-formyluradl, which is not affected by the presence of abasic sites and 5-formylcytosine, the modified cytosine counterpart of 5-formyluracil. In summar~ the innoxiousness, convenience, and cost-effidency of the 5-formyluracil phosphoramidite synthetic routine would promote the understanding of the epi~enetic role of this natural thvmidine modification.