欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
在在相关预防和临床的治疗的分子的水平表演伟人诺言的早癌症诊断。此处，我们开发了一个新奇方法在癌症房间表面上检测并且评估 biomarkers。基于 AS1411 aptamer 的优秀选择到指向的 nucleolin-overexpressed 房间，我们使用了一荧光灯有 pH 敏感的功能的探查由按反应与叠氮化物修改了标记 AS1411 为 aptamer。光谱 fluorophore naphthalimide 的特征有好 pH 依赖。由这样， nucleolin-overexpressed 房间能从正常被区别房间。进一步，这策略能也从邻近的良性的织物区别乳癌织物在修理福尔马林并且嵌入的 parrffin-(FFPE ) 织物标本。我们的方法有潜力在医药察觉被使用，这被考虑。
英文摘要：
Early cancer diagnosis in molecular level shows great promise in relevant prevention and clinical treatment. Herein, we developed a novel method to detect and evaluate biomarkers on cancer cell surface. Based on the excellent selectivity of AS1411 aptamer to targeted nucleolin-overexpressed cells, we used a fluorescent probe with a pH-sensitive function to label the AS1411 aptamer modified with azide by click-reaction. The spectral characteristics of fluorophore naphthalimide has a good pH dependence. By this way, nucleolin-overexpressed cell could be discriminated from normal cell. Further, this strategy could also discriminate the breast cancer tissue from the adjacent benign tissue in formalin-fixed and parrffin-embedded（FFPE） tissue specimens. It is considered that our method has the potential to be applied in medical detection.
同期刊论文项目
同项目期刊论文
Treatment Responses of Procaterol and CD38 Inhibitors in an Ozone-Induced Airway Hyperresponsiveness
Application of N-Halogeno-N-sodiobenzenesulfonamide Reagents to the Selective Detection of 5-Methylc
Graphene oxide-based fluorescent detection of DNA and enzymes using Hoechst 33258 and its use for du
Enzyme-directed pH-responsive exfoliation and dispersion of graphene and its decoration by gold nano
Polycations-functionalized water-soluble gold nanoclusters: a potential platform for simultaneous en
Highly sensitive electrochemiluminescent cytosensing using carbon nanodot@Ag hybrid material and gra
Carbon Dots Prepared by Hydrothermal Treatment of Dopamine as an Effective Fluorescent Sensing Platf
Incorporating Graphene Oxide and Gold Nanoclusters: A Synergistic Catalyst with Surprisingly High Pe
PEGylated hybrid ytterbia nanoparticles as high-performance diagnostic probes for in vivo magnetic r
Improvement of Photoluminescence of Graphene Quantum Dots with a Biocompatible Photochemical Reducti
A general approach using spiroborate reversible cross-linked Au nanoparticles for visual high-throug
Self-Assembled Peptide-Polyoxometalate Hybrid Nanospheres: Two in One Enhances Targeted Inhibition o
Lighting up left-handed Z-DNA: photoluminescent carbon dots induce DNA B to Z transition and perform
Triphenylamine pyridine acetonitrile fluorogens with green emission for pH sensing and application i
Hybridization chain reaction engineered dsDNA for Cu metallization: an enzyme-free platform for ampl
Bifunctionalized Mesoporous Silica-Supported Gold Nanoparticles: Intrinsic Oxidase and Peroxidase Ca
Near-infrared absorbing mesoporous carbon nanoparticle as an intelligent drug carrier for dual-trigg
Self-assembly of an organic-inorganic hybrid nanoflower as an efficient biomimetic catalyst for self
The Daxx/Atrx Complex Protects Tandem Repetitive Elements during DNA Hypomethylation by Promoting H3
Discovery of Small Molecules for Up-Regulating the Translation of Antiamyloidogenic Secretase, a Dis
Photosensitizer-Incorporated Quadruplex DNA-Gated Nanovechicles for Light-Triggered, Targeted Dual D
Aptamer-Directed Synthesis of Multifunctional Lanthanide-Doped Porous Nanoprobes for Targeted Imagin
Positional assembly of hemin and gold nanoparticles in graphene-mesoporous silica nanohybrids for ta
Single-layer tungsten oxide as intelligent photo-responsive nanoagents for permanent male sterilizat
Chemically exfoliated WS2 nanosheets efficiently inhibit amyloid beta-peptide aggregation and can be
An efficient nano-based theranostic system for multi-modal imaging-guided photothermal sterilization
Electrochemically and DNA-triggered cell release from ferrocene/beta-cyclodextrin and aptamer modifi
3D Graphene Oxide-Polymer Hydrogel: Near-Infrared Light-Triggered Active Scaffold for Reversible Cel
Catalytic Asymmetric 1,2-Addition of alpha-Isothiocyanato Phosphonates: Synthesis of Chiral beta-Hyd
Organocatalyzed Asymmetric 1,4-Addition of Azlactones to alpha,beta-Unsaturated Trichloromethyl Keto
Nanocomposite Incorporating V2O5 Nanowires and Gold Nanoparticles for Mimicking an Enzyme Cascade Re
Artificial Evolution of Graphene Oxide Chemzyme with Enantioselectivity and Near-Infrared Phototherm
Immunostimulatory oligonucleotides-loaded cationic graphene oxide with photothermally enhanced immun
Biocompatible and high-performance amino acids-capped MnWO4 nanocasting as a novel non-lanthanide co
Reduced Graphene Oxide Functionalized with a Luminescent Rare-Earth Complex for the Tracking and Pho
Upconverting Nanoparticles with a Mesoporous TiO2 Shell for Near-Infrared-Triggered Drug Delivery an
Luminescent Carbon Dot-Gated Nanovehicles for pH-Triggered Intracellular Controlled Release and Imag
Cerium oxide caged metal chelator: anti-aggregation and anti-oxidation integrated H2O2-responsive co
Mesoporous silica-encapsulated gold nanoparticles as artificial enzymes for self-activated cascade c
Ag Nanoparticle-decorated graphene quantum dots for label-free, rapid and sensitive detection of Ag+
Molecular crowding-facilitated synthesis of DNA-templated Ag nanoclusters with enhanced fluorescence
Qualitative and quantitative detection of methylation at CpG sites using the fluoresceind-GTP incorp
A novel aggregation-induced emission fluorescent probe for nucleic acid detection and its applicatio
A multi-stimuli responsive gold nanocage-hyaluronic platform for targeted photothermal and chemother
DNA-mediated biomineralization of rare-earth nanoparticles for simultaneous imaging and stimuli-resp
Towards intelligent bioreactor systems: triggering the release and mixing of compounds based on DNA-
Engineered, self-assembled near-infrared photothermal agents for combined tumor immunotherapy and ch
Anti-Biofouling Polymer-Decorated Lutetium-Based Nanoparticulate Contrast Agents for In Vivo High-Re
Cerium oxide nanoparticle: a remarkably versatile rare earth nanomaterial for biological application
Nucleoside Triphosphates as Promoters to Enhance Nanoceria Enzyme- like Activity and for Single- Nuc
Application of N？Halogeno？N？sodiobenzenesulfonamide Reagents to the Selective Detection of 5？Methylc
A convenient method for selective detection of 5-hydroxymethylcytosine and 5-formylcytosine sites in
Opposing enantiomers of tartaric acid anchored on a surface generate different insulin assemblies an
Array-Based Sensing of Proteins and Bacteria By Using Multiple Luminescent Nanodots as Fluorescent P
Ultrasensitive Telomerase Activity Detection in Circulating Tumor Cells Based on DNA Metallization a
Coupling exonuclease III with DNA metallization for amplified detection of biothiols at picomolar co
Transition-metal-substituted polyoxometalate derivatives as functional anti-amyloid agents for Alzhe
Visual detection of telomerase activity with a tunable dynamic range by using a gold nanoparticle pr
One-step nucleotide-programmed growth of porous upconversion nanoparticles: application to cell labe
Non-Enzymatic-Browning-Reaction: A Versatile Route for Production of Nitrogen-Doped Carbon Dots with
Label-free Electrochemiluminescent Enantioselective Sensor for Distinguishing between Chiral Metallo
Neuropeptide S interacts with the basolateral amygdala noradrenergic system in facilitating object r
Antinociceptive Effects of Central Administration of the Endogenous Cannabinoid Receptor Type 1 Agon
Reduced Graphene Oxide Upconversion Nanoparticle Hybrid for Electrochemiluminescent Sensing of a Pro
Discrimination between 5-hydroxymethylcytosine and 5-methylcytosine in DNA by selective chemical lab
Symmetric cyanovinyl-pyridinium triphenylamine: a novel fluorescent switch-on probe for an antiparal
Unfolding and Conformational Variations of ThrombinBinding DNA Aptamers: Synthesis, Circular Dichroi
Light Controlled Reversible Inversion of Nanophosphor-Stabilized Pickering Emulsions for Biphasic En
Multiconfi gurable Logic Gates Based on Fluorescence Switching in Adaptive Coordination Polymer Nano
Reduced graphene oxide supported chiral Ni particles as magnetically reusable and enantioselective c
Incorporating ATP into biomimetic catalysts for realizing exceptional enzymatic performance over a b
Multifunctional upconverting nanoparticles for near-infrared triggered and synergistic antibacterial
Functionalized graphene as sensitive electrochemical label in target-dependent linkage of split apta
Heterogeneous Assembled Nanocomplexes for Ratiometric Detection of Highly Reactive Oxygen Species in
Graphene-Mesoporous Silica-Dispersed Palladium Nanoparticles-Based Probe Carrier Platform for Electr
Near-Infrared Light-Triggered Drug-Delivery Vehicle for Mitochondria-Targeted Chemo-Photothermal The
Near-Infrared Light-Encoded Orthogonally Triggered and Logical Intracellular Release Using Gold Nano
A highly sensitive nucleic acid stain based on amino-modified tetraphenylethene: the influence of co
A new fluorescent turn-on probe for highly sensitive and selective detection of sulfite and bisulfit
Fluorescent Strategy Based on Cationic Conjugated Polymer Fluorescence Resonance Energy Transfer for
Nanoceria-Triggered Synergetic Drug Release Based on CeO2-Capped Mesoporous Silica Host-Guest Intera
Gold nanocluster-based vaccines for dual-delivery of antigens and immunostimulatory oligonucleotides
Design, synthesis and biological evaluation of novel 7-alkylamino substituted benzo[a]phenazin deriv
Target-responsive DNA-capped nanocontainer used for fabricating universal detector and performing lo
Transmutation of Personal Glucose Meters into Portable and Highly Sensitive Microbial Pathogen Detec
Enzyme-regulated the changes of pH values for assembling a colorimetric and multistage interconnecti
Synthesis of Fluorinated and Nonfluorinated Graphene Quantum Dots through a New Top-Down Strategy fo
Upconversion nanoprobes for efficiently in vitro imaging reactive oxygen species and in vivo diagnos
A two-photon fluorescent probe for selective methylglyoxal detection and application in living cells
Asymmetric Vinylogous Michael Addition/Cyclization Cascade Reaction for the Construction of Diversel
Growth of Hydrophilic CuS Nanowires via DNA-Mediated Self-Assembly Process and Their Use in Fabricat
Ionic Liquid-Assisted Synthesis of Multicolor Luminescent Silica Nanodots and Their Use as Anticount
Catalytic Asymmetric [3+2] Cyclization Reactions of 3-lsothiocyanato Oxindoles and Alkynyl Ketones V
Highly Enantioselective Cascade Reaction Catalyzed by Squaramides: the Synthesis of CF3-Containing C
Antimicrobial activities and action mechanism studies of transportan 10 and its analogues against mu
Design, synthesis, and evaluation of new endomorphin analogs with enhanced central antinociception a
Development and Application of alpha-Heteroatom Ketones in Asymmetric Michael Reaction with beta-tra
Intermolecular Enantioselective Dearomatization Reaction of beta-Naphthol Using meso-Aziridine: A Bi
Cell penetrating peptide TAT can kill cancer cells via membrane disruption after attachment of campt
The improved blood-brain barrier permeability of endomorphin-1 using the cell-penetrating peptide sy
Specific recognition of guanines in non-duplex regions of nucleic acids with potassium tungstate and
DNA-mediated Construction of Hollow Upconversion Nanoparticles for Protein Harvesting and Near- Infr
Fluorescein Derivatives as Bifunctional Molecules for the Simultaneous Inhibiting and Labeling of FT
Convenient and multiplexed detection of microRNAs based on an exonucleation reaction by conformation
Antimicrobial peptide protonectin disturbs the membrane integrity and induces ROS production in yeas
Application of a C-C Bond-Forming Conjugate Addition Reaction in Asymmetric Dearomatization of beta-
Individual Surface-Engineered Microorganisms as Robust Pickering Interfacial Biocatalysts for Resist
One-step synthesized immunostimulatory oligonucleotides-functionalized quantum dots for simultaneous
A CuS-based chemical tongue chip for pattern recognition of proteins and antibiotic-resistant bacter
期刊信息
位置：