中文摘要：

对产微球茎菌（Microbulbifer sp.）ALW1发酵产褐藻胶裂解酶5 L罐发酵工艺进行优化,建立褐藻胶裂解酶的高产发酵工艺。结果表明：海藻酸钠质量浓度为1 g/L时效果最好,浓度减半或加倍都会使酶活力下降;25℃比20℃更利于产酶;p H值控制在7.5时比自然条件下发酵产酶高峰提前,产酶量变化不大;在此基础上,对罐上工艺进行中试放大,20 L发酵罐酶活力最高为57.0 U/m L,200 L罐酶活力最高为43.5 U/m L,500 L罐酶活力最高为38.3 U/m L,分别是小试水平的39.6%、30.2%、26.5%。

英文摘要：

Microbulbifer sp. ALW1 isolate was subjected to fermentation parameters optimization in attempt to increase the alginate lyase producing ability in a 5 L bioreactor. The results showed that the addition of 1 g/L alginate was better for enzyme production than double or half the concentration,and 25 ℃ was more conductive to enzyme accumulation than 20 ℃. In addition,when p H was controlled at 7. 5,the peak of alginate lyase yield occurred earlier than that at natural p H,but the yields showed little change. Based on the optimized conditions,the fermentation processes for alginate lyase production were scaled up and the maximum alginate lyase activities of 57. 0 U/m L,43. 5 U/m L,and 38. 3 U/m L were achieved in 20 L bioreactor,200 L bioreactor and 500 L bioreactor respectively,which were 39. 6%,30. 2% and 26. 5% of the small scale level.