中文摘要：

以重组大肠杆菌（ Esherichia. coli） 发酵获得的能特异性水解琼脂硫酸酯的重组芳香基硫酸酯 酶为研究对象,以酶活力为指标,利用单因素试验对喷雾干燥法制备琼脂硫酸酯酶酶制剂的工艺参数进行 优化,并对琼脂硫酸酯酶贮藏过程中各影响因素进行了研究.结果表明,最佳的喷雾干燥工艺条件为利 用麦芽糊精作为助干剂,海藻糖为保护剂,麦芽糊精质量分数25%,进风温度130℃,进料速度400 mL/h, 热风流量3.5 m^3/min,喷雾压力0 .25 MPa.然后对制备得到的琼脂硫酸酯酶酶制剂的贮藏稳定性进行考 察,发现温度、空气对琼脂硫酸酯酶固体酶制剂的贮藏稳定性影响较大.分别在4℃和28℃贮藏91d, 4℃贮藏的固体酶制剂的酶活力保留率为71. 6 %,而在28 ℃贮藏的固体酶制剂酶活力保留率为63%.真 空条件下28℃贮藏91 d,酶活力保留率为6 5 .2 %,而敞口条件下为38.3%.光照对琼脂硫酸酯酶酶制剂 贮藏也有一定的影响,4℃下避光保存91 d,酶活力保留率为72.3%;而不避光贮藏的酶活力保留率只有 48.2 %.说明,琼脂硫酸酯酶固体酶制剂适宜在4℃、真空、避光条件下贮藏.

英文摘要：

Arylsulfatase specifically hydrolyzing agar sulfate was obtained from recombinant Escherichia coli. Then, the spray-drying process for enzyme preparation was optimized by the single factor experiments. The optimum spray-drying conditions were determined as follows ： 25 % maltodextrin as drying aid, inlet air temperature 130℃,hot air flow rate 3. 5 m^3/min, feeding rate 400 mL/h , and spray pressure 0. 25 MPa, respectively. Then, the appropriated storage method of enzyme preparation was investigated. It showed that the facts of temperature, and air had great influence on the storage stability of enzyme preparation. The solid enzyme was stored at 4℃ and 28℃ for 91 d separately, and the residual enzyme activities were 71. 6 % and 63%, respectively. The activity of solid enzyme remained 65. 2 % kept at 28℃ with sealed for 91 d, while the rediual activity of exposed enzyme was 38. 3%. In addition, the storage stability of enzyme preparation also was affected by light. The activity of solid enzyme remained 72. 3 % after stored at 4℃ in dark place for 91 d,whereas the rediual activity of exposed enzyme was only 48. 2%. Based on the experimental results, the solid enzyme was suited to be stored at 4℃,vacuum and dark place.