中文摘要：

目的 利用图形视觉诱发电位（PVEP）观察Wistar大鼠视皮层对不同空间和时间频率视觉刺激的反应调谐特性.方法 实验研究.8周龄Wistar大鼠15只,麻醉后于初级视皮层对应位置开颅并于硬脑膜表面慢性埋置记录电极,采用同样方法于鼻骨处埋置参考电极.分别于术后第3、5、7、9天行PVEP检测.采用图形棋盘格刺激,空间频率分别为0.01、0.02、0.04、0.08 cpd,时间频率分别为l、2、4、6 Hz.采用Matlab及SPSS软件对数据进行处理和统计学分析,比较不同时间、空间频率对大鼠PVEP幅度和潜伏期的影响,以及测量值的个体间变异和重复测量的变异性.结果 0.01、0.02、0.04、0.08 cpd空间频率下,P100波振幅分别为（29.87±10.37）、（31.92±10.98）、（28.46±6.18）、（20.71 ±6.54） μV,差异有统计学意义（F=3.725,P=0.018）;P100波潜伏期分别为（96.25±12.12）、（95.73±15.13）、（101.75±8.11）、（125.58±18.32） ms,差异有统计学意义（F=12.187,P =0.000）.1、2、4、6 Hz时间频率下P100波振幅分别为（30.71 ±8.25）、（29.75±4.76）、（25.79±8.51）、（19.63±6.00） μV,差异有统计学意义（F=6.115,P=0.001）;P100波潜伏期分别为（102.58±16.09）、（101.75±10.32）、（104.25±12.51）、（102.67±13.59）ms,差异无统计学意义（F=0.074,P =0.974）.空间频率为0.04cpd,时间频率为2 Hz时,PVEP的P100波振幅和潜伏期的个体间变异系数最小,而各种刺激条件下重复性测量的变异系数总体上无显著改变.结论 在适宜刺激和记录条件下可获得Wistar大鼠稳定可靠的PVEP记录.Wistar大鼠PVEP的P100波振幅的空间频率、时间频率调谐均表现为低通特性.空间频率对Wistar大鼠PVEP的P100波潜伏期的影响较为显著,而时间频率不影响PVEP的P100波潜伏期.

英文摘要：

Objective To observe the spatiotemporal frequency tuning of pattern visual evoked potentials in Wistar rats.Methods Experimental study.15 Wistar rats of 8 weeks old were used in this study.Recording electrodes were chronically inserted into the skull without piercing the dura at the corresponding sites to the primary visual cortex under anesthesia.Reference electrodes were inserted into the nose bone in the same way.Pattern visual evoked potentials were recorded on the 3rd,5th,7th and 9th postoperation days.The visual stimuli were chessboard patterns with spatial frequency of 0.01,0.02,0.04 or 0.08 cpd,and temporal frequency of 1,2,4 or 6 Hz,respectively.Data were processed and statistically analyzed with Matlab and SPSS.The amplitudes and latencies of pattern visual evoked potentials under different spatial and temporal frequencies,the inter-individual variations and the repeated measurement variations were compared.Results With the increase of spatial frequencies,the P100 amplitudes of PVEPs were gradually reduced,which were （29.87 ±10.37） μV （0.01 cpd）,（31.92 ±10.98） μV （0.02 cpd）,（28.46 ±6.18） μV （0.04 cpd） and （20.71 ±6.54） μV（0.08 cpd）,respectively,with significant difference among groups （F =3.725,P =0.018）,and the P100 latencies of PVEPs were gradually elongated,which were （96.25 ± 12.12） ms （0.01 cpd）,（95.73 ± 15.13） ms （0.02 cpd）,（101.75 ± 8.11） ms （0.04 cpd） and （125.58 ± 18.32） ms （0.08 cpd）,respectively,with significant difference among groups （F =12.187,P =0.000）.With the increase of temporal frequencies,the P100 amplitudes of PVEPs were gradually reduced,which were （30.71 ± 8.25） μV （1 Hz）,（29.75 ± 4.76） μV （2 Hz）,（25.79 ± 8.51） μV （4 Hz） and （19.63 ± 6.00） μV （6 Hz）,with significant difference among groups （F =6.115,P =0.001）,and there was no change of P100 latencies,which were （102.58 ± 16.09） ms （1 Hz）,（101.75 ± 10.32） ms （2 Hz）,（104.25 ±12.51） ms