中文摘要：

目的研究豹皮樟总黄酮（TFLC）抗肝纤维化作用并探讨作用机制。方法SD大鼠随机分为6组：正常组、模型组、TFLC组（100、200、400m异／kg）和秋水仙碱组（0．1mg/kg）。除正常组外，采用50％CCl4花生油溶液（1m1／kg）皮下注射，每周2次，连续12周复制肝纤维化模型。治疗组于造模第7周开始灌胃给予相应的药物。第12周末处死老鼠，分离血清，比色法检测谷丙转氨酶（ALT）、谷草转氨酶（AST）的含量，放免法检测透明质酸（HA）、层粘连蛋白（LN）、Ⅳ型胶原（CⅣ）、Ⅲ型前胶原氨端肽（PmNP）的含量。肝组织石蜡切片HE染色。采用RT-PCR法检测肝组织中TGF-β1、CTGFmRNA的表达。结果TFLC（200、400mg／kg）组血清中ALT、AST、HA、LN、CⅣ、PⅢNP的水平下降，肝纤维化程度明显减轻，肝组织中TGF-β1、CTGFmRNA的表达明显减少。结论TFLC有抗肝纤维化的作用，这可能与其能够抑制肝纤维化大鼠肝组织中TGF-β1、CTGFmRNA的表达有关。

英文摘要：

Objective To investigate the anti-hepatic fibrosis effect and partly mechanisms of total flavonoids of litsea coreana level（TFLC） in rats with hepatic fibrosis. Methods SD rats randomly divided into six groups [ （ the normal, the model, the TFLC （100 ,200 ,400 mg/kg） and the colchicin（0. 1 mg/kg） groups]. The model of hepatic fibrosis was induced by injecting 50% CC14 （1 ml/kg） twice weekly for 12 weeks. From the 7th week, all the therapeutic groups were treated with the TFLC （100,200,400 mg/kg） and the colchicin（0. 1 mg/kg） respectively for 6 weeks. At the 12nd week, the levels of ALT, AST, HA, LN, C Ⅳ and P III NP in serum were measured. HE staining was used to evaluate the degree of hepatic fibrosis. The mRNA expression of TGF-β1 and CTGF in liver tissue were detected by RT-PCR. Results TFLC（200,400 mg/kg） not only reduce the levels of ALT,AST,HA,LN, C IV and P HI NP in serum significantly, and the degree of hepatic fibrosis , but also decreased the mRNA expression of TGF-β1 and CTGF in liver tissues obviously. Conclusion TFLC has anti-hepatic fibrosis effect, which might be associated with inhibiting the TGF-β1 and CTGF mRNA expression of liver tissues in hepatic fibrosis rats.