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中文摘要：
以马铃薯多酚氧化酶为对象,研究金属离子对该酶活性的影响.实验结果表明,Li＋、K＋、Ba2＋和Mg2＋对酶活力几乎没有影响,Ca2＋和Na＋对酶活力有微弱的抑制作用,Cu2＋则表现出较强烈的抑制作用,其IC50为0.248 mmol/L.Al3＋、Fe3＋、Cd2＋、Co2＋、Mn2＋和Zn2＋则对马铃薯多酚氧化酶有不同程度的激活作用.本研究为探讨多酚氧化酶的催化活性和催化机理提供理论基础.
英文摘要：
In this paper, we studied the effects of some metal ions on the activity of polyphenol oxidase purified from potato. The results showed that Li^＋ , K^＋ , Ba^2＋ and Mg^2＋ had no effects on the enzyme activity. Ca^2＋ and Na^＋ showed a slight inhibition. Cu^2＋ could strongly inhibit the enzyme and the IC50 value was estimated to be 0. 248 mmol/L. Al^3＋ ,Fe^3＋ ,Cd^2＋ ,Co^2＋ ,Mn^2＋ and Zn^2＋ activated the activity of polyphenol oxidase. This paper laid the foundation for the studies of the enzyme activity,catalytic mechanism of poly-phenol oxidase.
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