中文摘要：

目的研究扶正透毒祛毒复方加味青蒿鳖甲汤对髓系微小残留白血病（MRD—L）患者骨髓CD34＋细胞源树突状细胞（DC）的影响。方法用Ficoll离心法分离急性髓系MRD—L患者骨髓单个核细胞（BMNC），采用免疫磁珠分选出CD34＋细胞，并培养扩增，用不同浓度中药含药血清联合细胞因子进行体外诱导培养DC，倒置显微镜下观察DC的形态学特征，流式细胞术检测DC表面分子CD83、CD80、CD86、CDla、HIA—DR的表达。诱导转化成熟的DC分别激发异体、自体T细胞，MTr法检测激发后的T细胞在不同效靶比时对人白血病细胞株l〈562的杀伤作用。结果各中药含药血清联合细胞因子培养髓系MRD—L患者CD34’9d后均能促进CD34’分化为形态特征典型的DC，各中药联合细胞因子组、细胞因子组均明显上调CD83、CD80、CD86、HIA—DR的表达，与胎牛血清及空白兔血清对照组比较有统计意义（P〈0．01），中剂量及低剂量含药血清组能促进CD1a的表达提高，与高剂量组、细胞因子组比较有差异（P〈0．01），其余各组无明显差异。DC激发T细胞杀伤K562细胞，各中药联合细胞因子组的杀伤率均高于细胞因子组、胎牛血清组和空白兔血清组，其中中剂量和低剂量组又明显优于（P〈0．01）或优于（P〈0．05）细胞因子组、胎牛血清组和空白兔血清组。T细胞的杀伤作用随效靶比的增高而增强，正常T细胞与患者T细胞对K562细胞的杀伤作用无明显差异（P〉O．05）。结论加味青蒿鳖甲汤能促进髓系MRD—L患者CD34＋细胞向DC转化。

英文摘要：

Objective To study the Qinghao Biejia Tang on CD34 ＋ derived influence of dendritic cells Fuzheng Toudu Qudu Fufang combining modified （DC） in the patients with minimal residual disease in myelogenous leukemia （MRD-L）. Methods Bone marrow mononuclear separated by using Ficoll centrifugation in the immuno-magnetic mircobeads and cultured for patients with MRD-L. CD34 ＋ cells amplification to get DC, which was cells （ BMNC ） were were isolated by using induced by medicated serum of Chinese medicinal in different concentrations in vitro. The morphologic characteristics of DC were observed with the inverted phase contrast microscope, and the expressions of DC surface molecules, CD83, CDs0, CD86 ,CDIa and HLA-DR, were detected by using flow cytometry. Mature DC was induced for activating T lymphocytes. The kill effect of activated T lymphocytes on K562 at different ratio of effectors to target cells was detected by using methyl thiazolyl tetrazolium test （MTY）. Results CD34 ~ was promoted by different medicated serum combined cytokines to differentiate into DC with typical morphology after being cultured for 9 days. The expressions of CD83, CD80, CDs6 and HLA-DR increased in high-dose ＋ cytokines group, mid-dose ＋ cytokines group, low-dose ＋ cytokines group and cytokines group compared with FCS serum group and rabbit serum group （ P 〈 0.01 ）. The expression of CDla increased in mid-dose ＋ cytokines group and low-dose ＋ eytokines group compared with high-dose ＋ cytokines group and cytokines group （ P 〈 0.01 ）, and there were no significant difference among other groups. Cytotoxicity of T lymphocytes to K562 cells were activated by DC. The kill rates of K562 in high- dose ＋ eytokines group, mid-dose ＋ cytokines group and low-dose ＋ cytokines group were all higher than those in cytokines group, FCS serum group and rabbit serum group, and the kill rates in mid-dose ＋ cytokines group and low-dose ＋ cytokines group were higher or significantly higher than those in cytoki