中文摘要：

【目的】研究扶正透毒祛毒复方（加味青蒿鳖甲汤）中药含药血清对髓系微小残留白血病（minimal residual disease in leukemia,MRD-L）患者CD34＋细胞源树突状细胞（DC）诱导培养的不同阶段血清中白细胞介素-2（IL-2）、可溶性白细胞介素-2受体（sIL-2R）的含量变化,探讨扶正透毒祛毒复方影响MRD-L患者CD34＋细胞源DC诱导及生物学效应的机制。【方法】将急性髓系白血病缓解期（AML-CR）患者骨髓经Ficoll密度梯度离心获得骨髓单个核细胞（BMNC）后,用免疫磁珠阳性选择法提取CD34＋细胞,培养扩增后用不同浓度中药含药血清与细胞因子进行体外培养诱导DC,培养过程中观察细胞的形态,流式细胞仪检测DC表面CD83、CD80、CD86、CD1a、人白细胞DR抗原（HLA-DR）的表达。在培养的第0天、第6天和第9天采用酶联免疫吸附（ELISA）法分别检测、比较各组血清中IL-2、sIL-2R的含量。【结果】（1）各中药含药血清联合细胞因子组能促进CD34＋细胞分化为形态特征典型的DC,并高表达CD83、CD80、CD86、HLA-DR等DC的表面标志,与胎牛血清及空白兔血清组比较差异有统计学意义（P〈0.01）,中药中剂量及低剂量含药血清组能促进CD1a的表达提高,与中药高剂量组、细胞因子组比较差异有统计学意义（P〈0.01）。（2）IL-2含量：含药血清制成后（第0天）,各中药含药血清组均高于空白兔血清组;各中药含药血清联合细胞因子组第9天、第6天均高于第0天,中药中剂量联合细胞因子组第9天显著高于第6天（P〈0.01）,中药高剂量联合细胞因子组第9天高于第6天（P〈0.05）;在同一时间内,各中药含药血清联合细胞因子组均高于空白兔血清组。（3）sIL-2R含量：含药血清制成后（第0天）,各中药含药血清组均低于空白兔血清组;各中药含药血清联合细胞因子组第9天均显著低于第0天、第6天（P〈0.01）,中药高剂量?

英文摘要：

Objective To study the influence of serum containing Fuzheng Toudu Qudu Recipe, a Chinese formula with the actions of supporting healthy qi to expel and remove toxicity, on serum levels of interleukin 2 （IL-2） and soluble interleukin 2 receptor （sIL-2R） at different stages of CD34＋ derived dendritic cells （DC） of patients with minimal residual disease of myelogenous leukemia （MRD-L） , and to explore the biological mechanism of Fuzheng Toudu Qudu Recipe in promoting CD34＋ to transform into DC in MRD-L patients. Methods Bone marrow mononuclear cells （BMMC） were separated from the bone marrow of acute myeloid leukemia patients at complete remission stage by using Ficoll centrifugation. CD34＋ cells were isolated by using immuno-magnetic mircobeads method, and then were cultured with various concentrations of Chinese medicine medicated serum and cytokines in vitro for the induction of DC. The morphologic characteristics of DC were observed with the inverted phase contrast microscope, and the expression levels of DC surface molecules such as CD83, CD80, CD86, CDla and HLA-DR were detected by using flow cytometry. On culturing day 0, 6 and 9, serum levels of IL-2 and sIL-2R of each group were measured by enzyme-linked immunosorbent assay （ELISA）. Results （1） Chinese medicine medicated serum combined with cytokines was effective on promoting CD34~ to differentiate into DC with typical morphology, and inducing DC to have high expression of CD80, CD83, CD86 and HLA-DR, which differed from those in fetal calf serum （FCS） group and blank rabbit serum group （P〈0.01）. Middle- and low-dose combination groups increased expression of CDla, which differed from high-dose combination group and cytokines group （P〈0.01）. （2） Content of IL-2 in combination groups was higher than that in blank rabbit serum group on culturing day 0. In the combination groups, IL-2 was higher on culturing day 6 and 9 than that on culturing day 0. Middle and high-dose combination groups had higher IL-2