中文摘要：

瞄准：为了估计不同老鼠的粘附和侵略能力，在不同房间线在试管内改编了 H pylori 紧张并且在毒力因素 cagA 和休假 A 上调查他们的效果。方法：在不同上皮的房间线的不同 H pylori 紧张的坚持和侵略能力被庆大霉素保护试金检验。cagA 和休假 A 的空异种被直接 PCR 变化方法处理。在 H pylori 附件以后的不同房间线的词法变化被显微镜学检验。结果：坚持到和进房间的侵略的密度在试管内与在老鼠感染实验的那些不同。88-3887 紧张能入侵并且遵守比 SS1 和 X47 强壮的房间。所有测试紧张最好粘住并且在 SCG-7901 的侵略能力房间。减异种的 CagA 和休假 A 有象他们的野类型的一样的侵略和支持者能力。在测试的所有紧张和房间线，仅仅 AGS 房间与 88-3887 在接种以后有重要蜂鸟显型野类型。结论：主人房间和细菌在侵略和 H pylori 的粘附能力起重要作用。CagA 和 VacA 不与侵略的能力和在不同房间线在试管内的 H pylori 的粘附有关。

英文摘要：

AIM： To assess the adhesion and invasion abilities of different mouse adapted H py/or/strains in different cell lines in vitro and investigate their effects on the virulence factors cagA and vacA. METHODS： The adherence and invasion abilities of different N pylori strains in different epithelial cell lines were examined by the gentamycin protection assay. The null mutants of cagA and vacA were processed by direct PCR mutation method. The morphologic changes of different cell lines after N pylori attachment were examined by microscopy. RESULTS： The densities of adherence to and invasion into cells in vitro were different from those in the mouse infection experiments. 88-3887 strain could invade and adhere to cells stronger than SS1 and X47. All tested strains had better adhering and invasive abilities in SCG-7901 cell. CagA and vacA minus mutants had the same invasion and adherent abilities as their wild types. In all strains and cell lines tested, only AGS cell had the significant hummingbird phenotype after inoculation with the 88-3887 wild-type. CONCLUSION： Both the host cells and the bacteria play important parts in the invasion and adhesion abilities of Hpylori. CagA and VacA are not related to the ability of invasion and adhesion of Hpylori in different cell lines in vitro.