中文摘要：

目的比较研究脂肪干细胞和嗅鞘细胞以两种不同方式共培养后脂肪干细胞P75的阳性表达。方法以Transwell小室为共培养载体。无支架共培养组以脂肪干细胞接种于上室,嗅鞘细胞接种于下室,支架共培养组以脂肪干细胞联合水凝胶生物支架（BDTM Pura MatrixTM Peptide Hydrogel）接种于上室,嗅鞘细胞接种于下室。检测两组细胞共培养12 d后其脂肪干细胞P75的表达。结果共培养12 d后无支架共培养组和支架共培养组都有部分细胞表达P75,且支架共培养组表达率更高。结论脂肪干细胞在嗅鞘细胞生长微环境中能向嗅鞘样细胞分化。

英文摘要：

Objective To observe the effects of olfactory ensheathing cells（OECs） and hydrogel on the differentiation of adipose-derived stem cells into OEC-like cells.Methods Transwell insert cell culture plate was used for co-culture experiments.Adipose-derived stem cells were placed in the upper chamber with hydrogel and OECs in the lower.In control group there was only adipose-derived stem cells in the upper chamber.After co-culture for 12 days the adipose-derived stem cells were tested if they expressed the antigenic phenotypes（P75） of OECs.Results OECs and hydrogel promoted differentiation of adipose-derived stem cells into OEC-like cells.The proportion of OEC-like cells was increased in experimental group as compared with control group.Conclusion OECs and hydrogel promote differentiation of adipose-derived stem cells into OEC-like cells.