中文摘要：

探索移植神经干细胞的移植的目的用 4.7T 先生系统并且到学习与 superparamagnetic 氧化铁(SPIO ) 和 bromodeoxyuridine (Brdu ) 共同标记有在 ischemic 老鼠的免疫组织化学的方法的房间区别。方法老鼠神经干细胞(NSC ) 与 SPIO 共同标记由 poly-L-lysine 调停了， bromodeoxyuridine (BrdU ) 与中间的服的动脉吸藏(MCAO ) 被移植进老鼠大脑的未受影响的方面。在星期 1， 2， 3， 4， 5，和 6 在 MCAO 以后，标记的房间的移植被 MRI 监视。在星期 6 点，老鼠被打死，他们的大脑织物根据 MRI 显示并且使遭到了到普鲁士的蓝染色并且 immunohistochemical 染色观察移植 NSC 的迁居和区别的移植房间的迁居地点被切。三个星期在移植以后结果，从标记的 NSC 的迁居导出的线性 hypointensity 区域被 MRI 在邻近注射地点的宫体 callosum 观察。在移植以后的六个星期，线性 hypointensity 区域沿着语料库 callosum 向中线被移动。MRI 调查结果被在在移植以后的星期 6 点的标本的普鲁士的蓝染色并且 immunohistochemical 染色证实。Flourescence 共同标记 immunohistochemical 方法证明移植 NSC 能区分进星形细胞和神经原。结论 MRI 能以一种动态、非侵略的方式在移植以后监视标记 SPIO 的 NSC 的移植。移植进老鼠能在移植的过程期间区分进星形细胞和神经原的 ischemic 的 NSC。

英文摘要：

Objective To explore the migration of transplanted neural stem cells co-labeled with superparamagnetic iron oxide （SPIO） and bromodeoxyuridine （Brdu） using the 4.7T MR system and to study the cell differentiation with immuno-histochemical method in ischemic rats. Methods Rat neural stem cells （NSCs） co-labelled with SPIO mediated by poly-L-lysine and bromodeoxyuridine （BrdU） were transplanted into the unaffected side of rat brain with middle cerebral artery occlusion （MCAO）. At weeks 1, 2, 3, 4, 5, and 6 after MCAO, migration of the labelled cells was monitored by MRI. At week 6 the rats were killed and their brain tissue was cut according to the migration site of transplanted ceils indicated by MRI and subjected to Prussian blue staining and immunohistochemical staining to observe the migration and differentiation of the transplanted NSCs. Results Three weeks after transplantation, the linear hypointensity area derived from the migration of labelled NSCs was observed by MRI in the corpus callosum adjacent to the injection site. Six weeks after the transplantation, the linear hypointensity area was moved toward the midline along the corpus callosum. MRI findings were confirmed by Prussian blue staining and immunohistochemical staining of the specimen at week 6 after the transplantation. Flourescence co-labelled immunohistochemical methods demonstrated that the transplanted NSCs could differentiate into astrocytes and neurons. Conclusion MRI can monitor the migration of SPIO-labelled NSCs after transplantation in a dynamical and non-invasive manner. NSCs transplanted into ischemic rats can differentiate into astrocytes and neurons during the process of migration.