中文摘要：

目的：检测Ets家族转录因子ER81在白消安模型小鼠睾丸及附睾中的表达水平,探讨其对精原细胞增殖和分化的影响。方法：成年小鼠腹腔注射白消安10 mg.kg-1,分别在注射后第0、3、5、8、10和18天取睾丸和附睾,半定量RT-PCR分析ER81 mRNA的相对表达量。结果：在睾丸组织内,与第0天比较,ER81的表达量在第5天显著降低（P〈0.01）,随后基本恢复;在附睾组织内,与第0天比较,ER81表达量于第8天显著降低（P〈0.05）,并随后恢复。结论：转录因子ER81可能对精原细胞的分化有调节作用。

英文摘要：

Objective To investigate the expressions of Ets family transcription factor ER81 in testis and epididymis of busulfan-treated mice,and explore its function on the proliferation and differentiation of spermatogonia.Methods Busulfan peritoneal injection（10 mg·kg-1） was performed and mouse testis and epididymis were collected on the 0th,3rd,5th,8th,10th,18th days after injection,respectively.The mRNA expression levels of ER81 in samples were analyzed by semi-quantitative RT-PCR.Results Compared with the 0th day,the expression of ER81 in testis was significantly decreased on the 5th day（P0.01） and then recovered gradually.In epididymis,compared with the 0th day,the expression of ER81 on the 8th day was significantly decreased（P0.05）.Conclusion ER81 may modulate the differentiation of spermatogonia.