中文摘要：

目的采用RP-HPLC法同时测定骨疏丹颗粒剂中新北美圣草苷、柚皮苷、淫羊藿苷、欧前胡素、蛇床子素5种活性成分。方法采用Diamonsil C18色谱柱（250 mm×4.6 mm, 5 μm），以乙腈-水为流动相，梯度洗脱，流速1.0 mL·min^-1，柱温为室温，检测波长285 nm。结果各个成分的峰面积与其质量浓度的线性关系良好（r〉0.999 0），加样回收率为95.8 %-98.6 %。结论该方法简单、准确、重现性好，可为全面评价复方骨疏丹颗粒剂的质量提供依据。

英文摘要：

Objective To determine the contents of the neoeriocitrin, icariin, osthole, naringin, imperatorin in Gushudan granules （ traditional Chinese medicine ）. Methods Diamondsil TM C18 （250 mm × 4.6 mm, 5 μm） column was used with a mobile phase of acetonitrile-water; the flow rate was 1.0 mL·min^- 1 ; the detection wavelength was set at 285 nm. Results The linearity was obtained with good result （r〈0.999 0）. The average recoveries of five active constitutes in Gushudan granules were all in 95.8%- 98.6%, and the RSD were less than 2.1%. Conclusion The validated method has the advantages of simplicity, precision and reliability, allowing quality control of Gushudan granules.