中文摘要：

目的：研究降钙素基因相关肽（CGRP）对大鼠血管平滑肌细胞中细胞周期蛋白依赖性激酶2（cyclin-dependent kinase2，CDK2）和细胞周期蛋白E（CycIinE）的影响，为阐明CGRP抑制血管平滑肌细胞增殖的细胞周期机制提供实验依据。方法：培养大鼠胸主动脉血管平滑肌细胞，分别用CGRP或／和10％FBS处理细胞。噻唑蓝比色法（MTT）观察CGRP对10％FBS诱导大鼠血管平滑肌细胞增殖的影响；流式细胞术分析细胞周期；Western blot检测CDK2和Cyelin E的表达。结果：CGRP能抑制10％FBS诱导的血管平滑肌细胞增殖，升高G0／G1。期细胞百分比，降低S＋G2：＋M期细胞百分比，抑制细胞内CDK2和Cyclin E表达。结论：CGRP能通过阻滞细胞周期由G0／G1，期进入S期而抑制血管平滑肌细胞增殖，其作用机制可能与降低CDK2和Cyclin E表达有关。

英文摘要：

AIM： To study the effect of calci- tonin gene-related peptide （CGRP） on cyclin-de- pendent kinase 2（CDK2） and Cyclin E of vascu- lar smooth muscle cell （VSMC）, and to elute the mechanism of inhibitory effect of CGRP on proliferation of VSMC in cell cycles. METH- ODS： VSMC were prepared from thoracic aortas of male Sprague-Dawley rat by explanting meth- od. The cell viability and cell cycle were deter- mined by MTT and Flow Cytometry, respective- ly. Western Blot was used to observe expres- sions of CDK2 and Cyclin E of VSMC. RE- SULTS： Pretreatment of VSMC with CGRP nificantly inhibited cells viability, decreased slg- theproportion of S phase and increased ratio of G0/ G1 that were induced by 10% FBS, simultane-ously, CGRP down-regulated the expressions of CDK2 and Cyclin E when cultured cells with 10% FBS at 12, 24 and 48 hours. CONCLU-SION= CGRP could inhibit the cell cycle of VSMC G0/G1 phase to S phase transition, by which the mechanism maybe involved in the de-crease expressions of CDK2 and Cyclin E.