中文摘要：

乙肝表面抗原结合蛋白（HBsAg binding protein,SBP）是本实验室发现的一种可以与乙肝表面抗原HBsAg特异性结合的人源蛋白,该蛋白已经被证实具有增强乙肝疫苗免疫效果的作用.目前,我们已经利用毕赤酵母表达系统获得了能够分泌表达SBP的毕赤酵母表达菌株.本研究通过对上述菌株的发酵产物进行超滤和亲和层析纯化,获得了一定量的高纯度重组SBP蛋白,并利用酶联免疫吸附检测（ELISA）法和表面等离子体共振（SPF）法分别对重组SBP进行了体内外生物学活性的初步检测,证实其具有与HBsAg结合的能力,并求得了二者之间的亲和常数.将重组SBP作为乙肝疫苗增效剂与乙肝疫苗共同免疫小鼠,SBP增效组小鼠与对照组相比,血清中HBsAg抗体显著升高,表明SBP在体液免疫方面对乙肝疫苗具有显著的增效作用.上述结果表明,SBP有望作为乙肝疫苗的免疫佐剂,在乙型肝炎防治方面有重要意义.

英文摘要：

HBsAg binding protain（SBP） was found in our previous study, which can specifically interact with HBsAg and whose ability on enhancing the immuno-response of HBV vaccine was proved. Now, with the Pichia pastoris expressing system, we have developed a Pichia pastoris strain expressing SBP secretively. In this work, we purified SBP through ultra-filtration and affinity chromatography, obtained high purified SBP. Then with the methods of ELISA and SPF, we characterized the biological activity of SBP, proved its affinity to HBsAg and calculated the affinity constant between them. After injection of SBP as an adjuvant of HBV vaccine to mice, compared with control group, SBP enhancing group showed an significant increase of HBsAb, proved the enhancing ability of SBP in humoral immunity. This work showed SBP can be used as an adjuvant of HBV vaccine, and was significant in Hepatitis B prevention and cure.