中文摘要：

把抵抗细菌 Deinococcus radiodurans 基于一台遗传上修改的收音机，我们构造了一个实时整个房间生物传感器在高度放射性的环境监视放射性和 genotoxicity。提高的绿荧光蛋白质(eGFP ) 被熔化到关键 DNA 损坏可诱导的消遣的倡导者从 D 的基因。radiodurans，和原生质标志带的作为结果的 DNA 碎片(PrecA-egfp ) 被介绍进 D。radiodurans R1 紧张将获得生物传感器紧张 DRG300。这设计紧张能表示 eGFP 蛋白质并且在消遣的正式就职产生荧光一个基因倡导者。在实时 D 基于在荧光紧张和蛋白质表示水平之间的关联。radiodurans 房间，当与象鲸鱼群那样的 DNA 损坏来源一起对待时，我们发现紧张 DRG300 的荧光正式就职以一种显著剂量依赖者方式反应妈放射和 mitomycin C。发现是令人鼓舞的广泛地，侦探范围和这的高敏感重建了紧张在前者与另外的整个房间生物传感器作比较报导。这些结果建议紧张 DRG300 是一个潜在的整个房间生物传感器构造一个侦探系统在实时在环境监视放射性、有毒的污染物质的生物危险。

英文摘要：

Based on a genetically modified radioresistant bacteria Deinococcus radiodurans, we constructed a real time whole cell biosensor to monitor radioactivity and genotoxicity in highly radioactive environment. The enhanced green fluorescence protein （eGFP） was fused to the promoter of the crucial DNA damage-inducible recA gene from D. radiodurans, and the consequent DNA fragment （PrecA-egfp） carried by plasmid was introduced into D. radiodurans R1 strain to obtain the biosensor strain DRG300. This engineered strain can express eGFP protein and generate fluorescence in induction of the recA gene promoter. Based on the correlation between fluorescence intensity and protein expression level in live D. radiodurans cells, we discovered that the fluorescence induction of strain DRG300 responds in a remarkable dose-dependent manner when treated with DNA damage sources such as gamma radiation and mitomycin C. It is encouraging to find the widely detective range and high sensitivity of this reconstructed strain comparing with other whole cell biosensors in former reports. These results suggest that the strain DRG300 is a potential whole cell biosensor to construct a detective system to monitor the biological hazards of radioactive and toxic pollutants in environment in real time.