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Functional analysis of helicase and three tandem HRDC domains of RecQ in Deinococcus radiodurans
  • ISSN号:1673-1581
  • 期刊名称:《浙江大学学报:B卷英文版》
  • 时间:0
  • 分类:Q78[生物学—分子生物学]
  • 作者机构:[1]Key Laboratory of Chinese Ministry of Agriculture for Nuclear-Agricultural Sciences, Institute of Nuclear-Agricultural Sciences Zhejiang University, Hangzhou 310029, China, [2]College of Life Sciences, Zhejiang University, Hangzhou 310029, China, [3]College of Life Sciences and Chemical Engineering, Huaiyin Institute of Technology, Huai'an 223001, China
  • 相关基金:Supported by the National Basic Research Program of China (Grant No. 2004CB 19604), National Science Fund for Distinguished Young Scholars (Grant No. 30425038), and Key Project from the National Natural Science Foundation of China (Grant No. 30330020)
中文摘要:

在优核质, Mre11-Rad50-Nbs1 (MRN ) 建筑群,住在发信号的 DNA 修理和检查点的十字路口,很快在跟随双海滨裂缝(DSB ) 正式就职的损坏地点形成突出的 foci。这建筑群执行 DSB 结束的起始的处理。在编码这建筑群的部件的基因的变化导致 DNA 损坏超敏性, genomic 不稳定性,弄短的 telomere,和异常成熟分裂。因此, MR 蛋白质高度在进化期间被保存。Mre11 和 Rad50 的细菌的 orthologs 分别地是 SbcD 和 SbcC 蛋白质。Deinococcus radiodurans,一极其无线电抵抗细菌,能修理几百导致放射的 DSB。SbcD 和 SbcC 蛋白质作为 Dr1921 和 Dr1922 基因的产品被识别。由直接反向取向的 insertional 傻瓜发育不全技术, sbcD 基因的混乱显著地增加房间“敏感到损坏代理人的 DNA 的各种各样的类型,例如电离放射,极端 iolet 照耀,氢过氧化物,和 mitomycin C。我们也提供 drSbcD 蛋白质在这个有机体在生长和 DNA 修理起一个重要作用的证据,特别在在到红外的 6000Gy 的细胞的暴露以后产生的 DSB 的修理。这些结果证明 drSbcD 蛋白质在 D 在 DSB 修理起一个重要作用。radiodurans。

英文摘要:

In eukaryotes, the Mre11-Rad50-Nbs1 (MRN) complex, which resides at the crossroads of DNA repair and checkpoint signaling, rapidly forms prominent foci at damage sites following double-strand break (DSB) induction. This complex carries out the initial processing of the DSB ends. Mutations in the genes that encode components of this complex result in DNA-damage hypersensitivity, genomic instability, telomere shortening, and aberrant meiosis. Therefore, the MR proteins are highly conserved during evolution. The bacterial orthologs of Mre11 and Rad50 are the SbcD and SbcC proteins, respectively. Deinococcus radiodurans, an extremely radioresistant bacterium, is able tO mend hundreds of radiation-induced DSBs. The SbcD and SbcC proteins were identified as the products of the Dr1921 and Dr1922 genes. Disruption of the sbcD gene, by direct reverse-orientation insertional mutagenesis technology, remarkably increases the cells' sensitivity to various types of DNA damaging agents, such as ionizing radiation, ultraviolet irradiation, hydrogen peroxide, and mitomycin C. We also provide evidence that the drSbcD protein plays an important role in both growth and DNA repair in this organism, especially in repair of DSBs generated after cellular exposure to 6000 Gy of IR. These results demonstrate that the drSbcD protein plays an important role in DSBs repair in Do radiodurans.

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期刊信息
  • 《浙江大学学报:B卷英文版》
  • 中国科技核心期刊
  • 主管单位:
  • 主办单位:浙江大学
  • 主编:
  • 地址:杭州玉古路20号,浙江大学学报《英文版》编辑部
  • 邮编:310027
  • 邮箱:jzus@zju.edu.cn
  • 电话:0571-87952276 87952331
  • 国际标准刊号:ISSN:1673-1581
  • 国内统一刊号:ISSN:33-1356/Q
  • 邮发代号:
  • 获奖情况:
  • 国内外数据库收录:
  • 被引量:323