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耐辐射球菌抗氧化系统中的一个新成员dr1127
  • ISSN号：0023-074X
  • 期刊名称：《科学通报》
  • 时间：0
  • 分类：Q945.78[生物学—植物学]
  • 作者机构：[1]浙江大学原子核农业科学研究所,杭州310029
  • 相关基金：国家杰出多年科学基金（批准号：30425038）、国家自然科学基金重点项目（批准号：30330020）、国家重点基础研究发展计划（批准号：2004CB719604）及国家自然科学基金（批准号：30670026）资助
作者： 吴媛媛[1], 田兵[1], 华跃进[1]
关键词： 耐辐射球菌, dr1127基因, 抗氧化
中文摘要：

构建了一个耐辐射球菌（Deinococcus radioduran）未知基因dr1127的功能缺陷株MT1127．γ射线和H2O2处理R1和MT1127的结果表明，dr1127基因的缺失影响了耐辐射球菌的辐射抗性和氧化抗性．测试了指数生长期和稳定期的R1株和MT1127株的细胞提取物活性氧自由基清除能力，结果发现，无论是超氧阴离子、H2O2还是羟自由基，MT1127株均表现为更弱的自由基清除能力，这些结果与前面的生存率实验结果相吻合，也在一定程度上帮助我们理解dr1127基因的功能，同时，在大肠杆菌BL21（DE3）系统中成功表达了dr1127基因产物，纯化得到46kD的蛋白产物，利用Fe^2＋-H2O2氧化损伤系统，检测到DR1127蛋白在体外保护DNA免受氧化损伤的功能，并测到该蛋白能通过结合双链DNA的方式来实现保护DNA的功能．本研究揭示了dr1127基因在氧化损伤胁迫中的功能，为耐辐射球菌庞大而又复杂的抗氧化系统增添了新的成员，也为深入研究耐辐射球菌的超强辐射抗性开辟了一个新的研究思路．

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期刊信息
  • 《科学通报》
  • 北大核心期刊（2011版）
  • 主管单位:中国科学院
  • 主办单位:中国科学院
  • 主编：周光召
  • 地址：北京东黄城根北街16号
  • 邮编：100717
  • 邮箱：csb@scichina.org
  • 电话：010-64036120 64012686
  • 国际标准刊号：ISSN：0023-074X
  • 国内统一刊号：ISSN：11-1784/N
  • 邮发代号:80-213
  • 获奖情况:
  • 首届国家期刊奖,中国期刊方阵“双高”期刊,第三届中国出版政府奖
  • 国内外数据库收录:
  • 美国化学文摘（网络版）,美国数学评论（网络版）,美国工程索引,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊（2004版）,中国北大核心期刊（2008版）,中国北大核心期刊（2011版）,中国北大核心期刊（2014版）,中国北大核心期刊（2000版）
  • 被引量:81792