中文摘要：

目的 探讨人肝再生增强因子（ALR）对钙结合蛋白S100A11启动子（S100A11p）转录的调节作用。方法 利用生物信息学技术确定S100A11P区域，聚合酶链反应（PCR）扩增S100A11p，克隆至真核报告载体pCAT3中，构建PCAT3-S100A11P报告载体；以该质粒转染HepG2细胞系，用酶联免疫吸附法（ELISA）检测报告基因编码产物氯霉素乙酰转移酶（CAT）的表达活性。并以人ALR的真核表达载体pcDNA3．1（-）-ALR共转染的HepG2细胞系，用酶联免疫吸附法（ELISA）检测CAT的表达活性。结果pCAT3-S100A11p在HepG2细胞中具有CAT的表达活性；以pcDNA3．1（-）-ALR和pCAT3-S100A11p共转染HepG2细胞，CAT表达活性是单独转染细胞组pCAT3-S100A11p的0．42倍。结论 所克隆的S100A11启动子有启动子的转录活性，ALR的转基因表达具有对S100A11基因的下调作用。

英文摘要：

Objective To investigate the effect of human augmenter of liver regeneration （hALR） on transcription of calgizzarin S100All gene promoter. Methods The sequence of calgizzarin S100All gene promoter was identified in GenBank by bioinformatics method and amplified by using HepG2 genome DNA as template by polymerase chain reaction （PCR）. The amplified product was cloned into pCAT3 reporter vector. The HepG2 cells were transfected by pCAT3-S100Allp, and the HepG2 cells cotranfected by pCAT3-S100Allp and pcDNA3.1 （ - ）-ALR. The chloramphenical acetyhransferase （CAT） activity was detected by an enzyme linked immunosorbent assay （ELISA） kit. Results The expression of CAT in cotransfection of pCAT3 - S100Allp and pcDNA3. 1 （ - ）-ALR was 0.42 times as high as that of pCAT3-S100Allp. Conclusion The S100Allp identified in this study has transcription activity. It is suggested that hALR can down - regulate the expression of S100All gene.