本试验选取6只装置永久性十二指肠和回肠瘘管、体重约55 kg的成年小尾寒羊母羊,按0(对照期)或0.9 mL/kg日粮(试验期)喂给福尔马林(各期n=6),收集十二指肠和回肠食糜,并进行消化代谢试验,以研究在粗料型日粮条件下口服福尔马林对绵羊消化代谢的影响。结果表明,口服福尔马林使绵羊的自由采食量增加13.0%(P〈0.01);前胃有机物(OM)和纤维素(Cel)的消化量分别增加21.9%(P〈0.01)和29.7%(P〈0.01);到达小肠的OM、粗蛋白质(CP)、微生物蛋白、过瘤胃蛋白、总氨基酸(TAA)和赖氨酸(Lys)量分别增加5.2%(P〈0.01)、21.5%(P〈0.01)、13.2%(P〈0.05)、29.4%(P〈0.05)、18.0%(P〈0.05)和21.8%(P〈0.05);小肠消化吸收的OM、CP、TAA和Lys量分别增加52.1%(P〈0.01)、34.7%(P〈0.01)、34.5%(P〈0.05)和32.8%(P〉0.05);日粮DM、OM、CP和Cel的(表观)消化率分别提高9.2%(P〈0.05)、9.2%(P〈0.05)、4.6%(P〈0.05)和5.8%(P〈0.05);氮、钙和磷保留量分别增加了28.3%(P〈0.01)、30.8%(P〈0.01)和9.5%(P〈0.05)。因此,在粗料型日粮条件下绵羊口服福尔马林可以提高瘤胃消化能力、提高采食量、增加小肠营养物质特别是氨基酸的消化吸收量、显著改善机体的营养状况。
6 of Small Tail Han ewes,fitted with permanent duodenum and ileum canula,with the body weight of 55 kg were orally administered with formalin at the dose of 0(control period) and 0.9 mL/kg ration(experimental period) in the condition of feeding the roughage diet.The collection of the chyme of duodenum and ileum and the experiment of digestion and metabolism were done,to study the effect of oral administration of formalin on the digestion and metabolism of sheep.The results showed that the oral administration of formalin in sheep increased the voluntary intake by 13.0%(P〈0.01);increased the digested OM and cellulose in fore stomach by 21.9%(P〈0.01)and 29.7%(P〈0.01)respectively;increased the OM,crude protein,microbe protein,by-pass protein,total amino acid and lysine arrived at small intestine by 5.2%(P〈0.01),21.5%(P〈0.01),13.2%(P〈0.05),29.4%(P〈0.05),18.0%(P〈0.05)and 21.8%(P〈0.05) respectively;increased the OM,crude protein,total amino acid and lysine absorbed by small intestine by 52.1%(P〈0.01),34.7%(P〈0.01),34.5%(P〈0.05) and 32.8%(P〈0.05) respectively;increased the apparent digestibility of the DM,OM,crude protein and cellulose of diet by 9.2%(P〈0.05),9.2%(P〈0.05),4.6%(P〈0.05) and 5.8%(P〈0.05) respectively;increased the retention of the nitrogen,calcium and phosphorus by 28.3%(P〈0.01),30.8%(P〈0.01) and 9.5%(P〈0.05) respectively.It is concluded that the oral administration of formalin of sheep fed with roughage diet increases the digestive ability of rumen,the intake,and the small intestine-absorbed nutrients,especially the amino acids,therefore modifies the nutritional status of sheep.