中文摘要：

目的：探讨神经调节素B受体（neuromedin B receptor,NMBR）在妊娠小鼠子宫平滑肌细胞中mRNA、蛋白时空表达的变化及其与分娩启动的关系和机制。方法：按妊娠阶段的不同,将小鼠分为未孕组、早孕组、中孕组、晚孕组、产时组和产后组,每组12例。应用半定量RT-PCR和Western免疫印迹技术分别检测子宫平滑肌中NMBR,IL-6和HSP70mRNA及蛋白表达;利用NoShift转录因子分析法检测NF-κB-P65的DNA结合活性,并分析各指标与分娩的关系及其相关性。结果：产时组NMBRmRNA的相对表达量显著高于未孕、早孕、晚孕、产后组（P〈0.05）,但与中孕组相比差异无统计学意义（P〉0.05）;产时组NMBR蛋白表达量显著高于其它各组（P〈0.01）。NF-κB-P65DNA结合活性产时组显著高于未孕、晚孕和产后组（P〈0.05）。IL-6mRNA表达产时组显著高于未孕、晚孕和产后组（P〈0.05）,其蛋白表达产时组亦显著高于未孕和产后组（P〈0.05）。HSP70mRNA表达量产时组显著高于未孕和产后组（P〈0.05）,HSP70蛋白的表达量产时组明显高于晚孕、未孕组（P〈0.05）。临产前后子宫平滑肌细胞P65DNA结合活性、IL-6mRNA的表达均与NMBRmRNA的变化呈正相关（r=0.40,P〈0.01;r=0.30,P〈0.05）;P65,HSP70mRNA的变化亦与NMBR的变化呈正相关（r=0.40,P〈0.01;r=0.49,P〈0.01）;但HSP70与P65无相关性。结论：子宫平滑肌细胞中NMBRmRNA和蛋白表达在临产时达最高峰。NMBR可借助NF-κB P65-IL-6通路、通过调节HSP70表达参与分娩发动和维持,但其对HSP70表达的影响不依赖P65途径。

英文摘要：

Objective To investigate the spatiotemporal expression of neuromedin B receptor（NMBR） in mice myometrium at different pregnant stages,as well as its mechanism and relation with parturition.Methods The pregnant mice were divided into no-pregnancy（NP）,early pregnancy（EP）,mid-pregnancy（MP）,late-pregnancy（LP）,parturition（PT）and postpartum（PP）groups（12 mice in each group）,according to pregnant stage.The mRNA and protein expression of NMBR,HSP70 and IL-6 were detected in myometrium in pregnant mice by semi-quantitative RT-PCR and Western blot,while NF-κB-P65 DNA binding activity was determined by NoShift transcription factor assay kits,respectively.Their relation with parturition was analyzed.Results The mRNA expression level of NMBR in the PT group was significantly higher than that in the NP,EP,LP and PP groups（P〈0.05）,but this difference was not observed in the MP group（P〉0.05）.The NMBR protein in PT group was significantly higher than that in the other 5 groups（P〈0.01）.NF-κB-P65 DNA binding activity at PT group was remarkably higher than that in the NP,LP and PP groups（P〈0.05）.The expression of IL-6 mRNA was significantly higher than that in the NP,LP and PP groups（P〈0.05）,its protein expression in PT and LP groups was significantly higher than that in the NP and PP groups（P〈0.05）.The expression of HSP70 mRNA in the PT group was significantly higher than that in the NP and PP groups（P〈0.05）,and the protein of HSP70 was significantly up-regulated in PT and PP groups compared with in NP and LP groups（P〈0.05）.The DNA-binding activity of P65 was positively ]elated to the mRNA expression of NMBR and IL-6（r=0.40,P〈0.01;r=0.30,P〈0.05）,so were positively correlated to DNA-binding activity of P65,mRNA expression of HSP70 and NMBR（r=0.40,P〈0.01;r=0.49,P〈0.01）.DNA-binding activity of P65 did not correlate with the mRNA expression of HSP70.Conclusion The mRNA and protein expressions of NMBR reach a peak at the onset of labor.NMBR