采用高效液相色谱法分别分析了4个不同厂家的11个感冒咳嗽颗粒样品、6种原药样品和1个原药混合样品,利用中药指纹图谱相似度软件及主成分分析投影判别法对实验数据进行分析。结果发现,不同厂家的感冒咳嗽颗粒存在显著性差异。使用主成分分析和相似度分析能对样品进行判别,结合原药及原药混合样品色谱图对感冒咳嗽颗粒中的有效成分进行研究,结果表明,该方法能够鉴别中药质量。
High performance liquid chromatography(HPLC) was employed to analyze eleven Ganmao Kesou granules samples from four manufacturers and six corresponding raw materials samples and one mixed sample of the raw materials with the use of similarity software and the technique of projection discrimination based on principal component analysis(PCA).The results showed that there was obvious difference among Ganmao Kesou granules from different manufacturers.More investigations in the chromatograms of raw materials samples and those of patent medicine samples were carried out.The proposed method developed can be effectively used to control the quality of Ganmao Kesou Granules and other traditional Chinese medicines.