中文摘要：

目的通过建立SD大鼠激素型骨质疏松模型，研究肝脏铁调素（hepcidin）基因表达在模型形成过程中的变化趋势，探讨其与骨质疏松形成的相关性。方法选SD大鼠80只，按随机法分为实验组和对照组，分别为40只。实验组于每周第1、4天肌肉注射地塞米松（0．25mg／100g），连续6周建立大鼠骨质疏松模型，对照组用等量生理盐水肌注。实验组和对照组都用RT-PCR法观察第14、21、24、28、31、35、38、42天肝脏铁调素的基因表达。结果与对照组相比，实验组大鼠肝脏铁调素基因表达在不同时间点存在明显不同：实验组第17天铁调素表达量明显上升，第21天至38天呈水平波动，第38天至42天又呈现显著上升。结论模型中肝脏铁调素基因的表达升高与骨质疏松的形成存在一定的联系，为临床上研究骨质疏松症的发病机制和诊断指标提供了新的依据。

英文摘要：

Objective We establish osteoporosis models of hormone SD rats, in order to detect the tendency of expression of liver hepcidin gene in the formation process of rats osteoporosis model and investigate associations between hepcidin and osteoporosis. Methods 80 SD rats were divided into experiment group and control group with 40 in each group. To i.m Dex 0.25 mg/100 g twice a week, so that the osteoporosis model in hormone was established after 6 weeks. To i. m the indetical ml saline with control group. Then we measured the expression of the liver hepcidin gene with RT-PCR on the 17th,21st,24th,28th,31st,35th,38th,42nd Day.Results Compared with control group, hepatic hepcidin gene expression of test group on different stage had obvious differences. From model made to 17th day, hepcidin mRNA expression was obviously increasing. From 21st day to 38th day, contents of hepcidin remained unchanged. After 38th day, it obviously rose. Conclusion The high expression of liver hepcidin in model rats liver may have some relations with osteoporosis formation. Hepcidin may become a new diadynamic criteria in abnormal change of bone mineralization, and offer a new proof to the clinical research of osteoporosis.