中文摘要：

目的研究STE12α基因对新生隐球菌形态学的影响。方法分别敲除血清A型和血清B型新生隐球菌菌株的STE12α基因,建立缺陷株,再将STE12α基因重新导入建立重建株。观察并比较野生株、STE12α基因缺陷株及重建株在体内、外孵育后菌落和菌落的形态学差异。结果 STE12α基因缺陷株组形成的菌落明显偏少,菌株直径偏小,荚膜发育不良,而重构株组这些方面的改变都得到了恢复。结论 STE12α基因对新生隐球菌的形态学改变有着重要的影响,可能直接影响其毒力。

英文摘要：

Objective To explore the effect of STE12α-gene on the morphology of Cryptococcus neoformans.Method The STE12α-gene was disrupted from Cryptococcus neoformans serotype A and serotype B to set up deficient strain.Then the gene was reconstituted into the allele locus of deficient strain.Morphological differences were studied in vitro and in vivo.Results Ste12α mutant strain exhibited an obvious defect in colony quantity,colony diameter and capsular size but recovered by reconstitution.Conclusion STE12α-gene play an important role in the morphology and virulence of Cryptococcus neoformans.