中文摘要：

目的：考察蛙凝素（Odorranalectin,OL）修饰对聚谷氨酸苄酯-聚乙二醇纳米粒（PBLG-PEG-NPs）材料的Calu-3细胞（人肺腺癌细胞）毒性和细胞摄取作用的影响。方法：碘氧化法制备蛙凝素修饰聚合物材料;以姜黄素（curcumin,Cur）为模型药物,采用乳化溶媒蒸发法制备聚谷氨酸苄酯-聚乙二醇纳米粒（PBLG-PEG-NPs）和蛙凝素修饰聚谷氨酸苄酯-聚乙二醇纳米粒（OL-PBLG-PEG-NPs）;MTT法考察三种纳米粒对Calu-3的细胞毒性;采用激光共聚焦显微镜对两种纳米粒的Calu-3细胞摄取作用进行定性观察。结果：给予高浓度（2 mg·mL-1）纳米粒培养Calu-3细胞时,细胞存活率大于75%。蛙凝素修饰纳米粒后其被细胞摄取的量从62.7%增加到了81.2%。结论：OL可用于黏膜给药载体的修饰,OL-PBLG-PEG-NPs细胞毒性低、生物相容性好,有望成为一种鼻腔粘膜给药优良载体。

英文摘要：

Objective： To investigate the cytotoxicity and cellular uptake efficiency of OL modified PBLG-PEG-NPs（PL-PBLG-PEG-NPs） compared with the non-modified one. Methods： OL-PEG-PLGA was synthesized using iodine oxidation method.MTT assay was used to evaluate the cytotoxicity of Poly（γ-benzyl-L-glutamate） block-ploy（ethylene glycol） nanoparticles（PBLG-PEG-NPs） to Calu-3 cells. The cellular uptake of nanoparticles was visualized by confocal laserscanning microscope. Results：The cytotoxicity results indicated that cell viability had reached 75 % while Calu-3 cells were incubated at high concentration of 2 mg·mL-1. After PBLG nanoparticles were odorranalectin-conjugated, the cellular uptake ratio raised from 62.7 % to 81.2 %. Conclusion OL-PBLG-PEG-NPs had low cell cytotoxicity and good biocompatibility. It might be used for nasal mucosa drug delivery.