中文摘要：

本文旨在考察比较3种聚氨基酸苄酯-聚乙二醇纳米粒（PXA—PEG—NPs）材料的Calu-3细胞（人肺腺癌细胞）毒性和细胞摄取作用，优选出一种PXA—PEG—NPs作为姜黄素鼻黏膜给药载体。以姜黄素（Cur）为模型药物，采用乳化溶媒蒸发法制备聚赖氨酸苄酯-聚乙二醇纳米粒（PZLL．PEG．NPs）、聚谷氨酸苄酯-聚乙二醇纳米粒（PBLG—PEG—NPs）和聚天冬氨酸苄酯-聚乙二醇纳米粒（PBLA—PEG—NPs）纳米粒。MTT法考察3种纳米粒的Calu．3细胞毒性：采用荧光倒置显微镜和流式细胞仪对3种纳米粒的Calu．3细胞摄取作用进行定性观察和定量测定。结果表明：给予高浓度（2mg·mL-1）的纳米粒时，未见细胞毒性。3种载姜黄素聚氨基酸纳米粒的细胞摄取量均显著高于姜黄素溶液组（姜黄素质量浓度5gg·mL-1），其中Cur-PBLG．PEG．NPs组最高；纳米粒的细胞摄取量随着孵育时间延长而增加，与纳米粒浓度呈正相关。可见，PXA．PEG—NPs能递送姜黄素进入细胞，PBLG-PEG．NPs有望成为一种安全的鼻黏膜给药优良载体。

英文摘要：

The aim of this paper is to compare the cytotoxicity and cellular uptake efficiency of three kinds of poly（b-benzyl-L-amino） block-poly（ethylene glycol） nanoparticles （PXA-PEG-NPs） using Calu-3 cells, and select one as a nasal drug delivery vector for curcumin （Cur）. Poly（7-benzyl-L-glutamate） block-poly（ethylene glycol） nanoparticles （PBLG-PEG-NPs）, poly（y-benzyl-L-lysine） block-poly（ethyleneglycol） nanoparticles （PZLL-PEG-NPs） and poly（7-benzyl-L-aspartate） block-poly（ethylene glycol） nanoparticles （PBLA-PEG-NPs） were prepared by emulsion-solvent evaporation method. MTT assays were used to evaluate the cytotoxicity of PXA-PEG-NPs against Calu-3 cells. The cellular uptake of nanoparticles was visualized by an inverted fluorescence microscope and quantified by a flow cytometer. The results indicated that even at high concentration of 2 mg-mL the three nanoparticles had no cytotoxicity on Calu-3 cells. Compared to the curcumin solution, the three curcumin-loaded PXA-PEG-NPs showed significantly higher cellular uptake efficiency on Calu-3 cells （at equal concentration of curcumin with 5 g.mL-1 Cur solution）, PBLG-PEG-NPs group was the highest. The cellular uptake increased with incubation time, and has positive correlation with nanoparticle concentration. In brief, PXA-PEG-NPs are conducive to delivery Cur into cells, and PBLG-PEG-NPs might be provided as agood nasal drug delivery carrier.