中文摘要：

目的：建立测定莪术中羟基异吉马呋内酯含量的方法，并比较不同产地莪术中羟基异吉马呋内酯的含量差异。方法：采用高效液相色谱法。色谱柱为KromasilC18（250mm×4．6mm，5μm），流动相为乙腈．水（梯度洗脱），流速为1．0ml／min，检测波长为214nm，柱温为25℃。结果：羟基异吉马呋内酯的质量浓度在1．47～14．68μg／ml范围内与其峰面积积分值呈良好线性关系（n=0．9997）；精密度、稳定性、重复性试验的RSD〈3％；平均加样回收率为95．31％～95．80％，RSD为0．07％～1．36％（n=3）。不同产地同品种、同产地不同时期莪术中羟基异吉马呋内酯的含量存在差异。结论：该方法分离度好、专属性强、重复性好、简便易行。可用于莪术中羟基异吉马呋内酯的含量测定，并能完善现行莪术药材的质量标准。

英文摘要：

OBJECTIVE： To establish the method for the content determination of hydroxyisogermafurenolide in Curcumae Rhi- zoma, and to compare the content difference of hydroxyisogermafurenolide in Curcumae Rhizoma from different habitats. METH- ODS： HPLC method was adopted. The separation was performed on Kromasil C18（250 mm×4.6 mm, 5 μm） column with mobile phase consisted of aeetonitrile-water（gradient elution） at the flow rate of 1.0 ml/min. The detection wavelength was set at 214 nm and column temperature was 25 ℃. RESULTS： The linear range of hydroxyisogermafurenolide were 1.47-14.68 μg/ml（r=0.999 7）. RSDs of precision, stability and reproducibility tests were all lower than 3%. The average recovery were 95.31%-95.80% （RSD were 0.07%-1.36%, n=3）. The contents of hydroxyisogermafurenolide in same types of Curcumae Rhizoma from different habitats were different from that of Curcumae Rhizoma from same habitats at different periods. CONCLUSIONS： The method is well-sepa- rated, specific, reproducible, simple and feasible. It can be used for the content determination of hydroxyisogermafurenolide in Curcumae Rhizoma and improve the present quality standard of Curcumae Rhizoma.