中文摘要：

本试验以主要在中性粒细胞中表达的磷酸二酯酶4B基因为靶基因,研究双黄连注射液的抗炎机制。采用半定量RT-PCR法检测双黄连注射液在不同时间对磷酸二酯酶4B基因表达的影响。结果发现双黄连注射液与中性粒细胞共孵育1 h时对磷酸二酯酶4B基因表达无明显的影响,当孵育到3 h时即可极显著抑制磷酸二酯酶4B基因的表达（P〈0.01）。本试验结果表明,双黄连注射液能在基因转录水平上抑制中性粒细胞内磷酸二酯酶4B基因的表达,提示其能使cAMP含量升高而抑制中性粒细胞等炎症细胞的活化,从而发挥抗炎作用。

英文摘要：

The influence of Shuanghuanglian Injection on phosphodiesterase 4B gene expression in neutrophils and it＇s anti-inflammatory mechanism in neutrophils were studied. The quantity of phosphodiesterase 4B gene expression was detected by semi-quantitative RT-PCR in different time to evaluated the influence of Shuanghuanglian Injection. Expression of phosphodiesterase 4B gene in neatrophils had no notable change after incubating with Shuanghuanglian Injection for 1 h, but significantly reduced （P〈0.01） after incubating with Shuanghuanglian Injection for 3 h. The results suggested that Shuanghuanglian Injection can inhibit phosphodiesterase 4B gene expression in neutrophils in certain time, making cAMP content rise and inhibiting activation of inflammatory cells such as neutrophils, consequently, to exert the anti-inflammation.