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Effect of pre-moxibustion on apoptosis and proliferation of gastric mucosa cells
  • ISSN号:1007-9327
  • 期刊名称:《世界胃肠病学杂志:英文版》
  • 时间:0
  • 分类:R246.1[医药卫生—针灸推拿学;医药卫生—中医临床基础;医药卫生—中医学] R245.8[医药卫生—针灸推拿学;医药卫生—中医临床基础;医药卫生—中医学]
  • 作者机构:[1]The Institute of Acupuncture, Moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China
  • 相关基金:Supported by the National Natural Science Foundation of China, No. 30572310, and the Major State Basic Research Development Program of China (973 program), No. 2005-CB523306-9.
中文摘要:

瞄准:与导致压力的溃疡在老鼠在胃的粘膜房间的 apoptosis 和增长上观察艾灸前的效果,并且分析在那些效果和热吃惊蛋白质 70 的表示(HSP70 ) 之间的关系。方法:六十只健康 Sprague Dawley 老鼠随机被分配进四个组,也就是组织 A, B, C 和 D。压力溃疡的动物模型走水路被建立沉浸和抑制应力。分别地,当那些在 Zusanli 和 Liangmen 点在组 C 收到了艾灸时,在组 A, B,和 D 的老鼠用作抑制,模型,和 non-acupoint 控制。Immunohistochemical 方法论被用来检测 HSP70 的表示, apoptosis 索引(AI,乘 10-6/mum2 ) 并且增长索引(PCNA-LI,乘 10-6/mum2 ) 。转变生长因素高山的粘膜表示哈(TGF-alpha ) 被放射性免疫测定检测。结果:在 Zusanli 和 Liangmen 点的艾灸显著地减少了胃的损害和胃的粘膜房间的 apoptosis,当显著地增加了时象胃的粘膜房间的增长一样的 TGF-alpha 和 HSP70 的粘膜表示。与组 A 相比,溃疡索引( UI )( 26.8 +/- 9.8 对 12.0 +/- 5.9 , P 【 0.01 ), AI ( 9.6 +/- 4.2 对 4.4 +/- 2.6 , P 【 0.05 )并且 HSP70 的表示( 9.6 +/- 4.2 对 4.4 +/- 2.6 , P 【 0.05 )显著地被增加,但是 TGF-alpha 的内容( 104.7 +/- 51.2 pg/mL 对 254.0 +/- 86.9 pg/mL , P 【 0.01 )并且 PCNA-LI ( 6.9 +/- 4.7 对 14.9 +/- 4.6 , P 【 0.05 )显著地在组 B 被减少。然而,溃疡索引价值(UI ) 和 AI 在与组 B 和 D 相比的组 C 显然是更低的(14.1 +/- 5.4 对 26.8 +/- 9.8 和 26.2 +/- 7.7, P 【 0.01; 3.0 +/- 1.6 对 9.6 +/- 4.2 和 8.2 +/- 5.2 , P 【 0.05 ,分别地),但是 TGF-alpha 的内容( 237.0 +/- 72.6 pg/mL 对 104.7 +/- 51.2 pg/mL 和 154.1 +/- 61.3 pg/mL , P 【 0.01 )并且 HSP70 的表示( 0.13 +/- 0.03 对 0.08 +/- 0.06 和 0.06 +/- 0.04 , P 【 0.05 )在组 C 是更高的。而且, PCNA-LI 比在组 B 在组 C 是显著地更高的(21.6 +/- 4.1 对 6.9 +/- 4.7, P 【 0.01 ) 。结论:在 Zusanli 和 Liangmen 点的艾灸?

英文摘要:

AIM: TO observe the effects of pre-moxibustion on apoptosis and proliferation of gastric mucosal cell in rats with stress-induced ulcer, and to analyze the relationship between those effects and the expression of heat-shock protein 70 (HSP70). METHODS: Sixty healthy Sprague Dawley rats were randomly assigned into four groups, namely group A, B, C and D. The animal model of stress ulcer was established by water immersion and restraint stress. The rats in group A, B, and D served as the restraint, model, and non-acupoint controls, respectively, while those in group C received moxibustion at Zusanli and Uangmen points. Immunohistochemical methodology was used to detect the expression of HSP70, apoptosis index (AI, × 10^-6/μm^2) and proliferation index (PCNA-LI, × 10^-6/μm^2). The mucosal expression of transforming growth factor α (TGF-α) was detected by radioimmunoassay. RESULTS: IVloxibustion at Zusanli and Liangmen points significantly decreased the gastric injury and the apoptosis of gastric mucosal cells, while markedly increased the mucosal expression of TGF-α and HSP70 as well as the proliferation of gastric mucosal cells. Compared with group A, ulcer index (UI) (26.8 ± 9.8 vs 12.0 ± 5.9, P 〈 0.01), AI (9.6 ± 4.2 vs 4.4 ± 2.6, P 〈 0.05) and expression of HSP70 (9.6 ± 4.2 vs 4.4 ± 2.6, P 〈 0.05) were significantly increased, but the content of TGF-α (104.7 ± 51.2 pg/mL vs 254.0 ± 86.9 pg/mL, P 〈 0.01) and PCNA-LI (6.9 ± 4.7 vs 14.9 ± 4.6, P 〈 0.05) were significantly decreased in group B. However, ulcer index values (UI) and AI were obviously lower in group C compared to groups B and D (14.1 ± 5.4 vs 26.8 ± 9.8 and 26.2 ± 7.7, P 〈 0.01, 3.0 ± 1.6 vs 9.6 ± 4.2 and 8.2 ± 5.2, P 〈0.05, respectively), but content of TGF-α (237.0 ± 72.6 pg/mL vs 104.7 ± 51.2 pg/mL and 154.1 ± 61.3 pg/mL, P 〈0.01) and expression of HSP70 (0.13 ± 0.03 vs 0.08 ± 0.06 and 0.06 ± 0.04, P 〈 0.05) were higher in group C. Furth

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  • 《世界胃肠病学杂志:英文版》
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