中文摘要：

目的探讨化痰祛瘀疏肝法对哮喘小鼠白介素13、18（IL-13／IL-18）失衡的干预作用。方法以10％卵蛋白／氢氧化铝[OVA／AL（OH）3]致敏、5％卵蛋白激发复制支气管哮喘小鼠模型，于实验第16～43d给药，第44d取材。用酶联免疫试验（ELISA）法检测肺泡灌洗液（BALF）中IL-13、IL—18浓度，实时定量PCR（RT—PCR）法检测肺组织匀浆中IL—13、IL-18mRNA表达。结果模型组小鼠IL一13浓度及mRNA均较空白组显著升高（P〈0．01）；IL-18浓度及mRNA均较空白组显著降低（P〈0．05）；各处理组IL-13浓度及mRNA均较模型组显著降低（P〈0．01），IL-18浓度及mRNA均显著升高（P〈0．05）；PCF组、SGPCG组IL-13浓度降低不及地米组（P〈0．01），PCF组、SGPCG组IL-18mRNA、IL-13／IL-18比值均与地米组相似（P〉0．05）；PCF组、SGPCG组问IL-13、IL-18浓度及mRNA、IL-13／IL-18比值差异均无统计学意义（P〉0．05）。结论化痰祛瘀疏肝法对哮喘小鼠升高的IL-13浓度及mRNA均有降低作用，对其降低的IL-18浓度及mRNA均有升高作用，对IL-13／IL-18比值的调节总体与地塞米松和化痰祛瘀平喘法相似。提示化痰祛瘀疏肝法对哮喘小鼠神经源性炎症相关的Thl／Th2免疫失衡有较为确切的干预作用。

英文摘要：

Objective To explore the intervention on IL - 13/IL - 18 imbalance in asthma mice treated with the therapy for resolving phlegm,removing stasis and soothing the liver. Methods 10% OVA/ AL（OH） 3 for sensitization and 5% egg albumen for excitation duplication were used to prepare bronchial asthma model in mice. Medication was given in the 16th -43rd days of experiment. The materials were collect- ed on the 44th day. ELISA was adopted to detect IL - 13 and IL - 18 concentrations in BALF. RT - PCR was used to detect the expressions of IL - 13 and IL - 18 mRNA in homogenate of lung tissue. Results IL - 13 concentration and its mRNA expression of mice in the model group were increased significantly as compared with those in the blank group（P 〈 0.01 ） and IL - 18 concentration and its mRNA expression were reduced significantly as compared with those in the blank group（P 〈 0.05 ）. In each treatment group,IL - 13 concen- tration and its mRNA expression were reduced significantly as compared with those in the model group（P 〈 0.01 ）and IL- 18 concentration and its mRNA expression were increased significantly（ P 〈 0.05 ）. The re- ducing of IL- 13 concentration in two herbal medicine groups was not good as the dexamethasone（DXM） group（P 〈0.01 ）. IL- 18 mRNA expression and IL- 13/IL- 18 ratio in the two herbal medicine groups were similar to those in DXM group（ P 〉0.05 ）. The differences in IL- 13 and IL- 18 concentrations andtheir mRNA expressions and IL - 13/IL - 18 ratio were not significant between the two herbal medicine groups （ P 〉 0.05 ）. Conclusion The therapy for resolving phlegm, removing stasis and soothing the liver re- duces the increased IL - 13 concentration and its mRNA expression in asthma mice and increases the reduced IL - 18 concentration and its mRNA expression. The overall regulation of IL - 13/IL - 18 ratio is similar to DXM. It is suggested that this therapy achieves the definite intervention on Thl/Th2 immune imbalance rele- vant with neuro~enic inflam