中文摘要：

目的：以异槲皮素为转化对象对肠内菌体外转化模型的转化稳定性进行考察。方法：按照先前建立的《人肠内细菌生物转化模型和标准操作规程》,采用人源不同及人源相同但培养代数不同的肠内菌建立体外转化模型,对异槲皮素进行生物转化。结果：在24 h内,采用不同人源的肠内菌建立的转化模型均可将异槲皮素转化为槲皮素,但是转化所需的时间存在差异;而随着培养代数的增加,肠内菌表现出转化效率增强的趋势。结论：本研究证明,按照《标准操作规程》建立的肠内菌体外转化模型比较稳定,所得实验结果具有重现性,可用于中药化学成分的肠内菌转化研究。

英文摘要：

Objective ：To investigate the stability of human intestinal bacterial biotransformation model using isoquercetin as the substrate of transformation. Methods ： The in vitro transformation model was established using the intestinal bacteria form different volun- teers,or different passages of the same volunteer in accordance with the ＂biotransformation model of human intestinal bacteria and its standard operating procedures＂ to transform isoquercetin. Results：Within 24 hours, all models established with the intestinal bacteria from different volunteers could transform isoquercetin to quercetin,and the transformation efficiency was inclined to increase with the in- crease of the number of culture passages. Conclusion ： The intestinal bacterial model established in accordance with the ＂standard operat- ing procedures＂ is stable and the results obtained with this model are reproducible,which demonstrats the suitability of this model for the investigation of the chemical constituents of Chinese medicinal materials.